摘要` 转录因子对很多下游基因的表达具有调控作用,在植物的生长发育、代谢及抵抗生物和非生物胁迫为中起着重要作用。随着全球环境的恶化以及人口增长的压力，植物抗逆机制和植物抗逆性的研究对于可持续发展的现代农业来说是至关重要的。植物经过长期的自然选择，不断进化以适应外界环境的变化，形成了对各种环境胁迫的适应性或抗逆能力。植物体通过感受环境胁迫信号，通过多种信号转导途径，诱导一系列相关基因的表达，参与响应胁迫反应，调节体内生理生化代谢，形成抗逆防御反应体系，以抵御环境胁迫对植物体的伤害。转录因子是一类能够与启动子区域顺式作用元件特异性结合的蛋白质，是植物中最大的基因家族之一。本实验通过构建携带 GAL4 顺式元件(GAL-UAS)双荧光素酶报告子载体和 GAL4BD(GAL4 的DNA Binding 结构域)融合表达目的转录因子(已知具有转录抑制活性的 OsMADS57 和转录激活活性的 OsDRAP1)的效应子载体，通过农杆菌介导的烟草瞬时转化体系对该系统进行验证，明确转录因子转录活性分析系统的灵敏性。研究结果将为进一步探究候选基因（转录因子）的生物学功能、转录调节机制研究提供技术支撑。关键词：植物；转录因子；双荧光素酶；转录活性；GAL41AbstractTranscription factors play a regulatory role in the expression of many downstream genes and play an important role in plant growth, metabolism and resistance to biological and abiotic stress. With the deterioration of the global environment and the pressure of population growth, the study of plant resistance mechanism and plant resistance is very important for sustainable modern agriculture. Plants have evolved over a long period of time to adapt to changes in the external environment, forming an adaptable or resistant ability to various environmental stress. By feeling the signal of environmental stress, through a variety of signal transduction channels, the plant body induces the expression of a series of related genes, participates in the response to the stress response, regulates the physiological and biochemical metabolism in the body, and forms a...
