不同浓度氯化钴诱导小鼠海马神经元细胞缺氧损伤模型的比较

不同浓度氯化钴诱导小鼠海马神经元细胞缺氧损伤模型的比较肖婷婷 1，王莉 1，曹相玫 2，倪新莉 1*(宁夏医科大学: 1.临床医学院，2.基础医学院，银川 750004 )基金项目:国家自然科学基金地区项目（81660229）; 宁夏科技创新领军人才项目（KJT2015017）* 通讯作者:倪新莉 电话: 13909586966; E-mail: 1284813176@qq.com;【摘要】 目的:研究不同浓度的氯化钴 (CoCl2)诱导小鼠海马神经元细胞（HT22）缺氧损伤模型的比较。方法:用不同浓度 (0、50、100、200μmol/L) CoCl2 建立 HT22 细胞缺氧模型。通过 HE 染色观察 HT22 细胞轴突生长的特征，CCK8 法检测 HT22 细胞体外增殖活性的变化， 同时，Western Blot 检测 HT22 细胞中 HIF-1α、tau5 的表达情况。结果: HE 显示CoCl2 可致 HT22 细胞轴突断裂，CCK8 显示 CoCl2 对 HT22 细胞增殖抑制作用表现出浓度依赖性。Western Blot 显示 CoCl2 浓度为 200μmol/L 时, HIF-1α、Tau5 蛋白表达与对照组相比差异有统计学意义。 结论：CoCl2 可剂量依赖性上调 HT22 细胞 HIF-1α 表达，致轴突损伤及 tau5 降低，建立细胞缺氧模型简单易行。【关键词】HT22 细胞; 氯化钴; 轴突；HIF-1αComparison of hypoxic injury models of hippocampal neurons induced by different concentrations of cobalt chloride in miceXiao Tingting1, Wang Li1,Cao Xiangmei2,Ni Xinli1 *( 1.School of Clinical Medicine, 2.School of Basic Medicine,，Ningxia Medical University Yinchuan 750004, China)[Abstract]: Objective: To compare the hypoxia injury model of mouse hippocampal neuron cells (HT22) induced by different concentrations of cobalt chloride (CoCl2). Methods: The hypoxia model of HT22 cells was established with different concentrations (0, 50, 100, 200 μmol / L) of CoCl2. The characteristics of axon growth of HT22 cells were observed by HE staining. The CCK8 method was used to detect the changes of HT22 cell proliferation activity in vitro. At the same time, Western Blot was used to detect the expression of HIF-1α and tau5 in HT22 cells. Results: HE s...