嗜热微生物的研究进展及应用

嗜热微生物的讨论进展及应用(6 页)Good is good, but better carries it.精益求精，善益求善。嗜热微生物的讨论进展及应用宜春学院 化学与生物工程学院 03 生物教育 张志刚指导老师：姜琼摘要 嗜热微生物是一类最适生长温度高于 45C 的微生物，嗜热酶是从嗜热微生物中分离得到的一类热稳定性酶，由于嗜热酶分子内部有很多氢键、二硫键及紧密而有韧性的空间结构的存在，所以嗜热酶在高温条件下具有很强的稳定性，高温反应活性，以及对有机溶剂、去污剂和变性剂的较强抗性，嗜热酶在许多方面都有广泛的应用。当前获得嗜热酶的方法主要有：直接从嗜热微生物中提取、利用基因工程技术在中温宿主中表达和利用定向分子进化技术筛选。目前酶的纯化主要采纳层析法、高效液相色谱法和电泳法等方法。由于环境恶化,能源危机,全球变暖等原因, 嗜热酶的开发和应用将出现更加诱人的前景。 关键词 嗜热微生物；嗜热酶；嗜热机制；应用Thermophilic microorganism research progress and applicationZhang Zhigang College of Chemistry and Bioengineering, Bioengineering (Biology education), YiChun University Advisor: Jiang QiongAbstract Thermophilic microorganisms is a type of microorganisms that the most suitable growth temperature is above 45C ,the thermophilic enzyme is a type of a thermostable enzyme separated from the thermophilic microorganism, because there is a lot of hydrogen keys and two-sulphur keys in the thermophilic enzyme molecule, so the thermophilic enzyme have a very strong stability in the hot condition, as well as there is a stronger fastness to organic solvent、 eradicator and denaturant,the thermophilic enzyme have an extensive application in many fields.The method which obtained the thermophilic enzyme includes three kinds at present: distilled directly from the thermophilic microorganism, expressed in the mild host making use of a genetic engineering technique and sieved with the directional molecule evolution technique. At present the enzyme purification methods are:c...