免疫学实验指导大连医科大学免疫教研室抗原抗体检测______________________________________4(一)免疫血清的制备与鉴定_____________________________________________4实验一、免疫血清的制备______________________________________________________4实验二、免疫血清的鉴定、纯化和保存__________________________________________6(二)血清学反应(Serological reaction)__________________________________81. 凝集反应(Agglutination reaction)__________________________________________9实验三、试管凝集实验(Tube agglutination test)—抗体效价测定___________________9实验四、玻片凝集试验(Slide agglutination test)________________________________102.沉淀反应(Precipitation reaction)___________________________________________11实验五、环状沉淀试验(Ring precipitation test)—血迹鉴定_______________________11实验六、单向免疫扩散法(Single radil immunodiffusion)—正常人群 IgG 正常值检测_12实验七、双向扩散法(Double diffusion)—抗原分析_____________________________14实验八、对流免疫电泳_______________________________________________________15实验九、免疫电泳(Immunoelectrophoresis)____________________________________163.补体相关试验____________________________________________________________17实验十一、总补体活性(CH50)的测定________________________________________17实验十二、补体介导的细胞毒试验_____________________________________________19(三)免疫标记技术____________________________________________________22实验十三、酶联免疫吸附试验——双抗体夹心法_________________________________22免疫细胞(Immunocyte)___________________________________________23(一)T 淋巴细胞检查__________________________________________________23实验十四、E 玫瑰花环形成试验(E Rosette Test)________________________________23实验十五、淋巴细胞转化试验(Lymphocyte Transformation Test, LTT)____________24实验十六、T 淋巴细胞 CD 亚群测定____________________________________________26实验十七、MTT 比色法测定活细胞数量及功能__________________________________28实验十八、白细胞介素 2 ...