SpantidepreventsupregulationofnitricoxidesynthaseexpressionandnitricoxideproductioninthespinalcordinducedbyfomaldehydeinratsLIWenBin,SUNXiaoCai,LIQingJun,LIShuQin,CHENXiaoLing(DepartmentofPathophysiology,InstituteofBasicMedicine,HebeiMedicalUniversity,Shijiazhuang050017,China)Abstract:AIMTostudytheeffectofspantide{[DArg1,DTrp7,9,Leu11]substanceP},anantagonistofsubstanceP(SP),ontheupregulationofnitricoxidesynthase(NOS)andnitricoxide(NO)inthespinalcordofratduringinflammatorypainandhyperalgesia.METHODSTheinflammatorypainandhyperalgesiawereinducedbysubcutaneousinjectionof5%fomaldehyde0.2mLintotherighthindpawoftherats.NOSexpressionlevelwasdeterminedusingNADPHdiaphorasehistochemistry,andNOproductionwasdeterminedbynitratereductasemethod.RESULTSThefomaldehydeinjectionsignificantlyin