第42卷第4期第484页2013年8月华中科技大学学报(医学版)ActaMedUnivSciTechnolHuazhongVol畅42No畅4P畅484Aug畅2013李明,男,1974年生,医学硕士,主治医师,E‐mail:pporal2010@163.com△通讯作者,Correspondingauthor,E‐mail:majingzhi2002@163.com内源性雌激素及其受体与非妊娠期牙龈瘤的关系李明1,逄爱慧1,李科珍2,杨焰1,魏婷1,马净植1△华中科技大学同济医学院附属同济医院1口腔医学中心,2妇产科,武汉430030摘要:目的研究雌激素受体ERβ在牙龈瘤瘤体以及同体正常牙龈的表达,探讨其与牙龈瘤临床病理学特征和外周血雌激素(E2)水平的关系。方法实验组33例牙龈瘤瘤体及同体正常牙龈,行苏木精‐伊红(HE)染色和ERβ免疫组化染色,酶联免疫吸附实验测定33例牙龈瘤组、24例健康对照组雌激素水平。结果牙龈瘤组E2水平(19畅34±3畅45)pg/mL略高于健康对照组(17畅60±2畅36)pg/mL,但两组间血清E2水平差异无统计学意义(P>0畅05)。牙龈瘤瘤体与同体正常牙龈ERβ表达差异有统计学意义(P<0畅05);牙龈瘤患者血清雌激素水平术后(18畅96±2畅89)pg/mL较术前(19畅34±3畅45)pg/mL略有降低,但差异无统计学意义(P>0畅05);等级资料的相关分析发现牙龈瘤组织中ERβ的表达分级与外周血E2水平不呈线性相关(rs=0畅3804,P>0畅05)。结论非妊娠期牙龈瘤组织存在着较高含量的ERβ,非妊娠期牙龈瘤的生长过程中可能存在一定程度的雌激素依赖性。外周血E2水平与ERβ表达无关。关键词:牙龈瘤;雌激素;雌激素受体;免疫组织化学中图分类号:R781畅4DOI:10畅3870/j畅issn畅1672‐0741畅2013畅04畅026AssociationbetweenEndogenousEstrogen/EstrogenReceptorsandEpulisinNon‐pregnantWomenLiMing1,PangAihui1,LiKezhen2etal1StomatologicalCenter,2DepartmentofGynecologyandObstetrics,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030,ChinaAbstractObjectiveToinvestigatetheexpressionofestrogenreceptorβ(ERβ)inepulisandnormalgingivaltissuesinnon‐pregnantwomen,andtheassociationoftheERβexpressionwithclinicopathologicalcharacteristicsandtheserumestradiol(E2)level畅MethodsThelevelofserumestradiol(E2)wasmeasuredbyenzymelinkedimmunosorbentassay(ELISA)in33patientswithepulisand24subjectswithnormalgingivaltissues畅TheERβexpressionwasdetectedin33epulistissuesnormalgingivaltissuesbyimmunohistochemistryaswell畅ResultsThelevelofserumE2was(19畅34±3畅45)pg/mLinpatientswithepulis,slightlyhigherthanthatinnormalcontrols[(17畅60±2畅36)pg/mL],andtherewasnosignificantdifference(P>0畅05)畅SignificantdifferenceintheERβexpressionswasnotedbetweenthetwogroups(P<0畅05)畅ThelevelofserumE2was(18畅96±2畅89)pg/mLinpatientswithepulisafteroperation,lowerthanbeforeoperation[(19畅34±3畅45)pg/mL],butnosignificantdifferencewasfound(P>0畅05)畅ThelevelofserumE2hadnopositivecorrelationwiththeexpressionofERβinpatientswithepulis(rs=0畅3804,P>0畅05)畅ConclusionTheERβexpressionwaselevatedinepulistissuesofnon‐pregnantwomen,sugges‐tingthedevelopmentofepulisinnon‐pregnantwomenisestrogen‐dependenttosomedegree畅Moreover,itwasfoundthatthese‐rumE2levelisnotrelatedtotheERβexpressioninpatientswithepulis畅Keywordsepulis;estrogen;estrogenreceptor;immunohistochemistry牙龈瘤来源于牙周膜及颌骨牙槽突的结缔组织。多认为是机械刺激及慢性炎症刺激形成的反应性增生物,并非是真性肿瘤。根据病理组织结构不同,牙龈瘤通常可分为肉芽肿型、纤维型及血...