.技术资料.专业整理.常用缓冲溶液的配制方法1甘氨酸-盐酸缓冲液（0.05mol/L）X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀释至200毫升pHXYpHXY2.02.42.62.85050505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量=75.07,0.2mol/L甘氨酸溶液含15.01克/升。2.邻苯二甲酸-盐酸缓冲液（0.05mol/L）X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHC1，再加水稀释到20毫升pH(20°C)XYpH(20C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23,0.2mol/L邻苯二甲酸氢溶液含40.85克/升3•磷酸氢二钠-柠檬酸缓冲液pH0.2mol/L0.1mol/LpH0.2mol/L0.1mol/LNa2HPO4柠檬酸Na2HPO4柠檬酸（毫升）（毫升）（毫升）（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量=14.98,0.2mol/L溶液为28.40克/升。Na2HPO4・2H2O分子量=178.05,0.2mol/L溶液含35.01克/升。C4H2O7•H2O分子量=210.14,0.1mol/L溶液为21.01克/升。.技术资料.专业整理.4•柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7H2O氢氧化钠（克）NaOH97%盐酸（毫升）HC1（浓）最终体积（升）①2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610①使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。5•柠檬酸-柠檬酸钠缓冲液（0.1mol/L）pH0.1mol/L柠檬酸（毫升）0.1mol/L柠檬酸钠（毫升）pH0.1mol/L柠檬酸（毫升）0.1mol/L柠檬酸钠（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6H8O7・H2O：分子量210.14,0.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5O7・2H2O:分子量294.12，0.1mol/L溶液为29.41克/毫升。6.乙酸-乙酸钠缓冲液（0.2mol/L）pH（18°C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）pH（18C）0.2mol/LNaAc（毫升）0.3mol/LHAc（毫升）2.60.759.254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60Na2Ac・3H2O分子量=136.09,0.2mol/L溶液为27.22克/升。7．磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2）0.2mol/L0.2mol/L0.2mol/L0.2mol/L.技术资料.专业整理.pHNa2HPO4（毫升）NaH2PO4（毫升）pHNa2HPO4（毫升）NaH2PO4（毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HPO4^2H2O分子量=178.05,0.2mol/L溶液为85.61克/升。Na2HPO4^12H2O分子量=358.22,0.2mol/L溶液为71.64克/升。Na2HPO4・2H2O分子量=156.03,0.2mol/L溶液为31.21克/升。（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15mol/L）pHmol/L/15Na2HPO4（毫升）mol/L//15KH2PO4（毫升）pHmol/L//15Na2HPO4（毫升）mol/L//15KH2PO4（毫升）4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00Na2HPO4・2H2O分子量=178.05，1/15M溶液为11.876克/升。KH2PO4分子量=136.09，1/15M溶液为9.078克/升。8•磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2MK2PO4+Y毫升0.2NNaOH加水...
