摘要核黄素，常称为维生素B2微溶于水，具有较强的生物活性，与人体的代谢活动密切相关。当其缺乏时，人体器官会发生一系列的病变导致疾病，如口角炎、唇炎、舌炎、眼结膜炎和阴囊炎等。现在对药品含量的检测方法有很多，荧光分析法一直广泛应用于药品分析，具有快速、准确、直观等优点，并且可以同时检测多种药物含量。本文通过荧光发射光谱法检测维生素B2类药品中核黄素的含量。在做好实验的前期工作后，配制核黄素标准液和维生素B2药品样液并检测其荧光强度。研究核黄素的激发谱和发射谱，确定最佳激励波长468nm和最佳荧光波长523nm。由得到的数据绘制核黄素标准液浓度与荧光强度关系的标准曲线，根据检测的维生素B2药品样液的荧光强度值，代入核黄素标准液曲线表达式得到样液核黄素浓度，进而计算出药品中核黄素的含量。实验结果令人满意，药品核黄素实际含量为5.3753mg/片，稍高于标示值。该方法具有成本低，检测简单快速、灵敏度高等特点。关键词：药物检测；核黄素；荧光分析；激发波长；发射波长；标准曲线ABSTRACTRiboflavin,whichiswidelyknownasvitaminB2,isslightlysolubleinwater.Itiscloselyrelatedtothebody'smetabolicactivitiesandpossessstrongbiologicalactivity.Withthelackofit,thehumanorganwillundergoaseriesofleisions,leadingtodiseasessuchasangularstomatitis,cheilitis,glossitis,conjunctivitisandoscheitis.etc.Nowtherearemanymethodsforthedetectionofdugs,Fluorescenceanalysishasbeenwidelyusedindruganalysis,withfast,accuracy,visualizationandotheradvantages,anditcansimultaneouslydetectavarietyofpharmaceuticalcomponents.InthispaperthejodwasdonethroughthefliorescencespectrometricanalysisofvitaminB2drugsinthedetectionofriboflavincontent.Afterpreparingthepreliminarywork,thenextstepispreparationofRiboflavinstandardsolutionandsamplesolution,andfinishthefluorescenceintensitydetectionofthem.Throughtheexperimentaldetectionoffluorescencespectra,theresultsshowtheoptimalemissionwavelengthof523nmandthebestexcitationwavelengthof468nm.ThenusingtestdatatodrawacurveaboutfluorescenceintensitytoRiboflavinsolutionconcenteation.Accordingtothedataofsample,Riboflavinsolutionconenteationofsamplecanbedeterminedthroughsubstitutingthedataintothecurvilinearexpression.,andRiboflavincontentintabletsisobtained.Theresultsweresatisfactory,theactualcontentofdrugriboflavinis5.3753mg/piece.,,whichisslightlyhigherthanindicatedvalueof5.0mg/piece.Themethodhasthecharacteristicsoflowcost,simpleandrapiddetectionandhighsensitivity.etc.Keywords:drugdetection;riboflavin;fluorescenceanalysis;excitationwavelength;emissionwavelength;standardcurve目录1.1课题研究背景.........................................................................11.2维生素.....................................................................................11.2.1维生素介绍....................................................................11.2.2维生素检测意义和方法................................................21.3荧光.........................................................................................31.3.1荧光介绍........................................................................31.3.2荧光机理........................................................................41.3.3荧光光谱........................................................................51.3.4荧光衰减........................................................................51.3.5荧光量子产率................................................................61.3.6荧光与分子结构............................................................61.3.7影响荧光因素...
