1 山东单县鸡大肠杆菌病病原的分离与鉴定及药敏试验王亚西南大学荣昌校区动物医学系，重庆荣昌 402460 摘要：鸡大肠杆菌感染是危害养鸡业的主要细菌性传染病，无菌采取单县各大鸡场中疑似大肠杆病死雏鸡肝、脾、肺等脏器病料，通过结合综合流行病学、临床学、病理学、生物化学和传染病学， 进行分离与鉴定，最终确诊为鸡大肠杆菌病。从部分养鸡场分离出的细菌进行药敏试验，通过药敏试验筛选出敏感药物，为治疗与预防提供理论依据和实际应用参数。关键词： 大肠杆菌；分离；鉴定；药敏试验The Separation,identification and Antimicrobial Susceptibility Test of Chicken Colibacillosis in Shanxian ,Shandong WANG Ya Department of Veterinary Medicine ,Southwest University, Chongqing, 402460 ,China Abstract: Chicken colibacillosis infection is the major infectious diseases in poultry industry. Sterilely resorting to visceral organs, such as livers ,spleens, lungs of illness, chicklings that are suspected colibacillosis of each large chicken places in Shanxian,by the means of combing with epidemiology,clinical, pathology, biochemistry and epidemiology,we can separate and identificate to diagnose whether it is colibacillosis or not. By the means of form dyeing and Biochemical tests and then antimicrobial susceptibility test on bacterium that is separated from part chicken places,we can screen some Sensitive drugs and find more effective proprietary to Provide theoretical basis and practical parameters of treatment and prevention.. Key words: Colibacillosis ； Separation and Identification ；Antimicrobial Susceptibility Test 引言鸡大肠杆菌病是由大肠埃希氏杆菌(Escherichiacoli，E.coli)某些血清型菌株引起的一类疾病的总称[1] 。2 大肠杆菌致病性菌株的致病因子主要由该病原体的三个特征所决定：侵袭力，感染力，致病性潜力。该菌主要有三个原类型，即菌体抗原，表面抗原，鞭毛抗原。该菌对外界的抵抗能力不强，一般 60℃15min 便可致死， 对一般消毒药的抵抗力也很弱，但常用的消毒药水均能将其杀死[...
