目录 前言..................................................................1 1 菌种的选育..........................................................2 1.1 菌种的选育及制备................................................2 1.2 诱变育种........................................................3 1.3 菌种的保藏......................................................5 2 培养基的设计及灭菌..................................................6 2.1 培养基的类型及功能..............................................6 2.2 培养基的成分....................................................6 2.3 培养基的设计及优化 ............................................7 2.4 培养基设计时注意的问题..........................................8 2.5 培养基灭菌......................................................8 3 空气灭菌............................................................9 3.1 过滤除菌流程及设备..............................................9 3.2 无菌空气的检查.................................................11 3.3 发酵罐灭菌.....................................................11 4 种子的扩大培养.....................................................12 4.1 实验室种子制备.................................................12 4.2 生产车间种子制备...............................................12 5 发酵过程的参数控制.................................................13 5.1 发酵过程的补料策略.............................................13 5.2 发酵过程pH 值的控制............................................13 5.3 发酵过程温度的控制.............................................13 5.4 溶氧的控制.....................................................14 5.5 最佳接种量.....................................................14 5.6 泡沫的影响及控制...............................................14 5.7 染菌的控制.....................................................14 6 下游加工...........................................................15 6.1 发酵液的预处理和固液分离.......
