精品文档---下载后可任意编辑AtBl-1 延缓 MeJA 诱导的叶片衰老的调控机制讨论的开题报告摘要：在植物的生长过程中，叶片衰老是一个不可避开的过程。为了探究植物叶片衰老调控机制，本文选取了水稻 AtBl-1 基因，讨论其在 MeJA诱导的叶片衰老过程中的作用机制。通过构建 AtBl-1 基因的植物表达载体，转化到拟南芥中，得到了稳定过表达 AtBl-1 基因的转基因拟南芥株系。利用叶绿素荧光图片及相关指标对转基因和野生型植株的叶片进行生理生化分析，发现过表达 AtBl-1 基因的转基因植株叶片的叶绿素荧光值更高，光合作用差异较小，衰老过程缓慢，说明 AtBl-1 基因为延缓叶片衰老发挥了重要作用。接下来，本文将采纳 RNA-seq 技术构建 AtBl-1基因的表达谱，对 MeJA 诱导的转基因和野生型植株进行比较分析，旨在阐明 AtBl-1 基因在 MeJA 诱导的叶片衰老过程中的作用机制。本讨论对揭示植物叶片衰老调控机制，提高作物的耐久性方面有一定的理论意义和实践价值。关键词：AtBl-1；MeJA；叶片衰老；生理生化分析；RNA-seqAbstract:Leaf senescence is an inevitable process in plant growth. In order to explore the regulatory mechanism of plant leaf senescence, this paper selected the rice AtBl-1 gene and studied its role in the process of MeJA-induced leaf senescence. By constructing a plant expression vector of the AtBl-1 gene and transforming it into Arabidopsis, a stable transgenic Arabidopsis line overexpressing the AtBl-1 gene was obtained. Physiological and biochemical analysis of the leaves of transgenic and wild-type plants was carried out using chlorophyll fluorescence images and related indicators. It was found that the chlorophyll fluorescence value of the overexpressing AtBl-1 gene transgenic plants was higher, the difference in photosynthesis was small, and the senescence process was slow, indicating that the AtBl-1 gene played an important role in delaying leaf senescence. Next, this paper will use RNA-seq technology to construct the expression profile of the AtBl-1 gene and compare the MeJA-induced transgenic and wild-type plants, aiming to elucidate the role of the AtBl-1 gene in the process of MeJA-induced leaf senescence. This study has certain theoretical significance and practical value in 精品文档---下载后可任意编辑revealing the regulatory mechanism of plant leaf senescence and improving the durability of crops.Keywords: AtBl-1; MeJA; leaf senescence; physiological and biochemical analysis; RNA-seq
