精品文档---下载后可任意编辑CsNMAPK 过表达对 NO3-胁迫下黄瓜幼苗生物生化特性的影响的开题报告摘要：黄瓜是一种重要的蔬菜作物，然而，NO3-的过量浸染常被认为是黄瓜的一种生物胁迫因素。本讨论通过构建 CsNMAPK（Cucumis sativus mitogen-activated protein kinase）基因过表达的黄瓜幼苗模型，探讨 CsNMAPK 在 NO3-胁迫下对黄瓜幼苗的生物生化特性的调控作用。结果表明，CsNMAPK 高表达显著抑制了 NO3-胁迫对黄瓜幼苗生长的负面影响，促进了幼苗的生长，增加了叶片的面积和数量，并改善了根系的系统发育。同时，CsNMAPK 高表达显著提高了 NO3-吸收和积累速度，增加了幼苗的叶绿素含量和光合速率，降低了 NO3-处理下黄瓜幼苗的丙二醛、过氧化物酶活性和脯氨酸含量，表明 CsNMAPK 提高了NO3-胁迫下黄瓜幼苗的耐受能力，降低了胁迫造成的细胞氧化损伤。因此，我们得出结论：CsNMAPK 过表达可以提高 NO3-胁迫下黄瓜幼苗的生长和代谢水平，增强其耐受能力，为黄瓜幼苗抵御环境胁迫提供了新的思路。关键词：CsNMAPK；黄瓜幼苗；NO3-胁迫；生物生化特性；耐受能力Abstract:Cucumber is an important vegetable crop, however, excessive NO3- infiltration is often considered as a biological stress factor for cucumber. In this study, we construct a cucumber seedling model with overexpressing CsNMAPK ( Cucumis sativus mitogen-activated protein kinase) gene to explore the regulatory effect of CsNMAPK on the biological and biochemical characteristics of cucumber seedlings under NO3- stress. The results showed that the overexpression of CsNMAPK significantly inhibited the negative effect of NO3- stress on the growth of cucumber seedlings, promoted the growth of seedlings, increased the area and quantity of leaves, and improved the systemic development of roots. At the same time, CsNMAPK overexpression significantly increased the NO3- absorption and accumulation rate, increased the chlorophyll content and photosynthetic rate of seedlings, and reduced the malondialdehyde, peroxidase activity and proline content of cucumber seedlings under NO3- treatment, indicating that CsNMAPK increased the tolerance of cucumber 精品文档---下载后可任意编辑seedlings under NO3- stress and reduced the oxidative damage caused by stress. Therefore, we conclude that CsNMAPK overexpression can improve the growth and metabolic level of cucumber seedlings under NO3- stress, enhance their stress resistance, and provide new ideas for cucumber seedlings to resist environmental stress.Keywords: CsNMAPK, cucumber seedlings, NO3- stress, biological and biochemical characteristics, stress resistance.
