精品文档---下载后可任意编辑hMLH1 基因启动子甲基化与非小细胞肺癌细胞顺铂耐药方面的讨论的开题报告Title: Investigation of hMLH1 Gene Promoter Methylation in Relation to Platinum Resistance in Non-Small Cell Lung Cancer CellsIntroduction:Non-small cell lung cancer (NSCLC) remains one of the leading causes of cancer-related mortality worldwide. Resistance to platinum-based chemotherapy is a common challenge in the treatment of NSCLC. Methylation of hMLH1 gene promoter has been reported to be associated with platinum resistance in various types of cancer. However, the role of hMLH1 promoter methylation in platinum resistance in NSCLC is not well established. Therefore, the aim of this study is to investigate the correlation between hMLH1 gene promoter methylation and platinum resistance in NSCLC cells.Methods:The study will be performed using NSCLC cell lines, where one group of cells will be sensitive to platinum while the other group will be selected for resistance to platinum. The expression of hMLH1 gene will be analyzed by quantitative real-time PCR and western blot, while the promoter methylation of the gene will be assessed by methylation-specific PCR. The sensitivity of cells to platinum will be evaluated by MTT assay, and apoptosis will be detected by flow cytometry.Expected results:We expect to find higher levels of hMLH1 promoter methylation in platinum-resistant NSCLC cells compared to sensitive cells. Furthermore, we predict that the overexpression of hMLH1 gene and reduction of promoter methylation could reverse platinum resistance and sensitize NSCLC cells to platinum chemotherapy.Conclusion:The results of this study may contribute to a better understanding of the mechanism underlying platinum resistance in NSCLC and provide a potential therapeutic target for improving the efficacy of platinum-based chemotherapy.
