精品文档---下载后可任意编辑鲤遗传连锁图谱构建及耐低温性状相关标记的筛选的开题报告【摘要】鲤是我国重要的经济鱼类之一，但其耐低温性状的遗传机制仍不清楚。本讨论拟构建鲤遗传连锁图谱，并从中筛选出与耐低温性状相关的标记。首先，选择适当的亲本组合，通过 RAD-seq 技术构建全基因组的 SNP 数据库，并利用群体分型分析软件对 SNP 数据进行分析和筛选，得到具有多态性且适合遗传连锁分析的 SNP 标记。然后，在低温适应的鲤群体和对比群体中分别测定其耐低温性状，并对两个群体的 SNP 标记进行基因型分析和比较。最后，利用单因素方差分析和 QTL 分析方法，筛选出与耐低温性状相关的 SNP 标记，并构建一个遗传连锁图谱。本讨论的结论将有助于深化探究鲤的低温适应机制，并为鲤的遗传改良提供基础数据和理论支持。【关键词】鲤；遗传连锁图谱；RAD-seq；耐低温性状；标记筛选【Abstract】The carp is one of the important economic fish in China, but the genetic mechanism of its tolerance to low temperature is still unclear. This study intends to construct a genetic linkage map of carp and screen out markers related to low temperature tolerance from it. Firstly, appropriate parental combinations will be chosen to construct a genome-wide SNP database by RAD-seq technology. The population genotyping analysis software will be used to analyze and screen the SNP data, and SNP markers with polymorphism and suitable for genetic linkage analysis will be obtained. Then, the low-temperature-adapted carp population and the control population will be measured for their low-temperature tolerance, and the genotype analysis and comparison of SNP markers in the two populations will be performed. Finally, using one-factor variance analysis and QTL analysis methods, SNP markers related to low-temperature tolerance will be screened out and a genetic linkage map will be constructed. The conclusion of this study will help to explore the low-temperature adaptation mechanism of carp and provide basic data and theoretical support for the genetic improvement of carp.精品文档---下载后可任意编辑【Keywords】Carp; genetic linkage map; RAD-seq; low temperature tolerance; marker screening
