ＰＣ R 设计引物时酶切位点旳保护酶寡核苷酸序列切割率％2 hr20 h ｒAc ｃ IGG ＴＣ G ＡＣ C ﻫC Ｇ GT Ｃ GACC Ｇ ﻫＣＣGGTC Ｇ A Ｃ CGG 0 ﻫ0 00 0 ﻫ0Afl IIICACA Ｔ GTG ﻫＣ CACA Ｔ G Ｔ GG ﻫＣCCACATGTGG Ｇ0 ﻫ>90 >９0０ ﻫ>90 ﻫ＞90Asc IG Ｇ CGCGCC AGGC Ｇ C Ｇ CCT ﻫTTGGCG Ｃ GCCAA＞90 >90 ＞90>90 ﻫ>90 ﻫ>90A ｖ a ICCCCG Ｇ GG ﻫＣＣＣＣ CGGGGG ﻫTC ＣＣ CC Ｇ GGGGA５０ >9 ０ ﻫ>9０>9０ ＞9０ >90BamH IＣＧ GAT Ｃ C Ｇ Ｃ GGGATC ＣＣ G C Ｇ C Ｇ GATCCG Ｃ G1 ０ >90 >9 ０2 ５ ﻫ>9０ ﻫ>9０Bg ｌ IICAGATCTG ﻫＧ AAGATC Ｔ TC ﻫGGAAGATCT ＴＣ C0 ﻫ5７ 250 ﻫ>90 ﻫ>9０Ｂｓｓ H IIG Ｇ C Ｇ CG Ｃ C ﻫAGG ＣＧＣＧＣ CT TTG Ｇ CGCGCC ＡＡ0 ﻫ0 ﻫ５０0 ﻫ0 ＞９0Ｂ st Ｅ IＩG Ｇ G Ｔ(A/T)AC Ｃ C010ＢｓｔＸ IAA Ｃ TGCAGA Ａ C Ｃ AA Ｔ GC Ａ TTGG AAAAC Ｔ GCAGC ＣＡ ATGCATT Ｇ G Ａ A CTGCAGAACCAA Ｔ GC Ａ TTGG Ａ T Ｇ CAT0 ﻫ25 250 ５0 ＞9０Cla IＣ A Ｔ CGATG ﻫＧ ATCGATC C Ｃ ATCGATGG Ｃ CCATCGA Ｔ G ＧＧ0 ﻫ0 ﻫ>90 500 ﻫ0 >90 50EcoR ＩＧ GA Ａ TT ＣＣ ﻫＣＧ G ＡＡ TTCC Ｇ ﻫＣCGGAA Ｔ TC Ｃ GG>9 ０ ﻫ>90 ﻫ>0９＞９0 >９0 ﻫ>９0Hae Ｉ IIGGG Ｇ C Ｃ CC AGC Ｇ GCCG Ｃ T TT ＧＣ GGCCGCA Ａ＞90 ﻫ＞90 ﻫ>0９>90 ﻫ>90 >90Hind IIIＣ AA ＧＣ T Ｔ G CCAA Ｇ CT Ｔ G Ｇ ﻫＣ CCAA Ｇ CTTGG Ｇ0 ﻫ０ ﻫ100 0 75Ｋ pn IGGGT Ａ CCC GGG Ｇ TACCC Ｃ ﻫＣＧ GGGTA Ｃ CC Ｃ G0 >90 >9 ０0 ﻫ>9０ ﻫ＞90Mlu IG Ａ CGCGTC ﻫＣ G Ａ CGC Ｇ TCG０ ﻫ2５0 ﻫ50Nco ICCCATG Ｇ G ＣＡＴ GCCAT ＧＧＣ AT Ｇ0 ５ 0０ 75Nd ｅ ＩCCAT Ａ T Ｇ G ﻫCC Ｃ AT Ａ TGGG ＣＧＣ CATATGG Ｃ G ﻫＧ G Ｇ TTT Ｃ ATA ＴGAA Ａ CCC GG Ａ A Ｔ TCCATATGGAATT Ｃ C ﻫGGGAATTCC Ａ TAT Ｇ G Ａ AT ＴＣ C Ｃ０ ﻫ０ ﻫ0 0 ﻫ5７ ﻫ5７０ 0 ０ ﻫ0 >9０ ﻫ>90Nhe IGGCTAG Ｃ C ﻫCG ＧＣ T ＡＧ CC Ｇ...
