摘 要桑黄是产多糖的药用真菌。本文以来自长白山的桑黄菌作为出发菌种，采纳 PDA 培育基进行菌种活化，先测出桑黄的生长曲线和桑黄胞内、外多糖的积累曲线，然后用单因素实验法分别对桑黄液体培育基的初始 pH 值、碳源以及氮源进行了讨论。结果表明：优化的碳源为玉米粉，氮源为黄豆饼粉，初始 pH为 5.5 ～ 6.5 。 优 化 培 育 基 的 具 体 配 方 为 ： 玉 米 粉 3% 、 黄 豆 饼 粉1% 、 KH2PO40.3% 、 MgSO40.15% 、 维 生 素 B120μg/100mL 、 维 生 素 B230μg/100mL、pH 在 5.5～6.5 之间。经过验证实验，在温度为 30℃、转速为 130r/min 的条件下培育桑黄，发酵 7 天后，优化培育基中发酵产生的桑黄菌丝体干重为 7.089g/L，比 PDA 培育基中桑黄菌丝体产量提高了 80.93%。关键词：桑黄 液体培育 菌丝体 多糖 AbstractPhellinus litenus is a medicinal fungus producing intracellular polysaccharide. In this article Phellinus litenus we used is from The Chang Bai Mountain. First， the Phellinus litenus was inoculumed on the PDA culture medium in order to active the fungus. Second， basing on the dates， the mycelia growth curve and production of intracellular and extracellular polysaccharide of Phellinus litenus was educed . Then ，by the method of the single factor experiment method to carry on a research about initial pH value、carbon source and nitrogen source .As a result ， the best carbon source of the media is corn flour， the most excellent nitrogen source is soybean powder and the fittest pH is ranged from 5.5 to 6.5.The optimized media is corn flour 3%，soybean powders1%， KH2PO4 0.3%， MgSO4 0.15%， vitamin B1 20μg/100mL and vitamin B2 30μg/100mL，besides， pH is 5.5 to 6.5. Under the condition of 30 ℃ ， 130 r/min ，after 7 days， 7.089g mycelia (dry wt) per L submerged culture of Phellinus linteus was obtained. That is ， the mycelium production by P.linteus has enhanced by 80...
