题 目:贵州两种苦丁茶黄酮含量及抗氧化活性比较 摘 要黄酮是一类化合物的总称，它具有抑菌、抗炎、抗肿瘤、抗 HIV、抗氧化、抗肿瘤等功效。在木姜子植物中，生物碱、黄酮类、甾体、木质素、内脂、挥发油等在医学研究上比较常用。黄酮易溶于乙醇等有机溶剂，根据相似相容原理，本实验采用乙醇热回流提法提贵州遵义余庆大叶苦丁茶和遵义余庆小叶苦丁茶（leaf of chinese holly ）中的总黄酮，以芦丁为对照品，以黄酮提取率和抗氧化活性进行试验，在试验的基础上通过测出贵州遵义余庆大叶苦丁茶和小叶苦丁茶黄酮含量的以及抗氧化活性的强弱，最终通过试验比较得出贵州两种苦丁茶总黄酮含量多少以及它们抗氧化的强弱。并采用紫外-可见分光光度法测定贵州两种苦丁茶提取液中总黄酮的含量，最后采用 DPPH 自由基清除率实验测定黄酮的抗氧化活性。得到试验结果如下:在大叶苦丁茶黄酮提取率为 1.356%，小叶苦丁茶黄酮提取率为 1.235%，大叶苦丁茶抗氧化活性为 75.3，小叶苦丁茶抗氧化活性为 73.6关键词：苦丁茶；回流提取，黄酮含量，抗氧化活性ABSTRACTFlavonoids are a class of compounds, which have antibacterial, anti-inflammatory, anti-tumor, anti HIV, anti-oxidation, anti-tumor effect. In the plant, the alkaloids, flavonoids, steroids, lignin, internal fat, volatile oil and so on are commonly used in medical research.Flavonoids soluble in organic solvents such as ethanol, according to the similarity principle of compatibility, the experiment of extracting Litsea Guizhou by ethanol extraction (Litsea cubeba (Lour.) Pers) total flavonoids, rutin, the extraction rate of flavonoids as index, ethanol concentration, solid-liquid ratio, extraction temperature single factor test and extraction time as factors, the orthogonal test to investigate the effects of ethanol concentration, extraction time, extraction temperature and solid-liquid ratio on the extraction rate of flavonoids on the basis of single factor test, finally through the orthogonal test the optimum extraction conditions of total flavonoids in Guizho...
