自体和异体血液在全血分离实验体系中对免疫系统的影响分析研究 临床医学管理专业VIP专享

【摘要】 目的：运用系统血液免疫反应分离实验体系，探讨在抗原激活的情况下异体血细胞对反应体系中细胞因子含 量水平影响的变化规律。方法：以 S180 作为激活抗原，加异体血细胞与自体血细胞对比，37℃孵育 1 h，取上清液用 ELISA 法测定 IL-8、TNF -α、IFN -γ 的分泌量。结果：IL-8 的分泌量异体红细胞实验组〔（133.92±135.85）%〕与自体红细胞对照组〔（37.93±27.74）%〕相比明显上升（P < 0.05）；TNF -α 的分泌量异体红细胞实验组 〔（8.31±3.57）%〕与自体红细胞实验组〔（16.55±10.38）%〕相比明显下降（P < 0.05）；IFN -γ 分泌量无明显差异。结论：异体红细胞对自体血液免疫反应细胞因子的分泌具有双向调控作用，提示异体输血对受者的血液免疫反应具有干扰和失平衡的不良影响。【关键词】 淋巴细胞；红细胞；抗原；免疫调控；细胞因子 Effects of Autogenetic and Allogenous Blood on Cytokines by Means of Natural Isolated Experimental System 〔Abstract〕 Objective Autogenetic and allogenous erythrocytes regulate the immunological reactions activated by antigens by means of natural isolated experimental system, to observe the changes of cytokines. Methods Using inactivated S180 as activating antigen and autologous plasma as reaction substratum, After incubation an hour in 37℃, supernate fluid is suspended and detected the change of IL-8、TNF -αand IFN -γ by ELISA assay. Results The secretion of IL-8 of allogenous erythrocytes experimental group〔(133.92±135.85)%〕is much higher than that of autogeneic erythrocytes control group〔(37.93±27.74)%〕 (P < 0.05), The level of TNF -α of allogenous erythrocytes experimental group〔(8.31±3.57)%〕is much lower than that of autogeneic erythrocytes experimental group〔(16.55±10.38)%〕(P < 0.05); the expression of IFN -γ 红细胞在天然免疫防御网络中发挥着重要作用，红细胞在补体系统天然免疫反应的参与下有黏附和调理抗原、清除循环免疫复合物、调控淋巴细胞表达免疫分子和细胞因子等作用。有实验证明在库存血中有多达 14 种以上炎性...