第1页共6页编号:时间:2021年x月x日书山有路勤为径,学海无涯苦作舟页码:第1页共6页史上最快最全的网络稳定批量下载,尽在:http://item.taobao.com/item.htm?id=9176907081癃开颗粒抗前列腺增生的药理毒理研究谢家骏1钱伯初2高崎1周光兴3蔡华芳2乔正东1成苗1(1.上海市中药研究所,上海201203;2.浙江省医学科学院药物所,浙江杭州310006;3.复旦大学,上海200032)关键词:癃开颗粒前列腺增生药效毒理小鼠大鼠犬摘要:目的:探讨ig癃开颗粒抗前列腺增生药效与毒性作用。方法:给小鼠ig癃开颗粒,测定正常幼年小鼠和丙酸睾丸素皮下注射或尿生殖窦植入诱导的前列腺增生模型动物前列腺指数、前列腺组织内DNA含量、血清酸性磷酸酶活性、精囊和睾丸湿重。以口服给药途径和常规实验方法,检测单次给药的小鼠最大给药量,考察3个月连续给药的Wistar大鼠或Beagle犬长期毒性反应。结果:ig癃开颗粒20、40g/kg(Qd×20d)能降低正常幼年小鼠前列腺的重量及该组织内DNA的含量;ig癃开颗粒10、20、40g/kg(Qd×10d)能抑制丙酸睾丸素引起的小鼠前列腺腹叶的增生;ig癃开颗粒20、40g/kg(Qd×30d)能抑制植入胎鼠尿生殖窦引起的小鼠前列腺腹叶的增生;ig癃开颗粒100g/kg(Qd×13w)能使大鼠前列腺呈轻度萎缩状态,腺上皮由柱状向扁平移生,腺腔变小,停药4周未见消失;癃开颗粒ig对小鼠的最大给药量为200g/kg。癃开颗粒给大鼠ig10、40、100g/kg或给Beagle犬ig12、60g/kg(Qd×13周),未发现有明显的毒性反应。结论:癃开颗粒口服能抑制前列腺增生且无明显毒性反应。所属专业:应用药理(中药药理)作者简介:谢家骏(1959~),男,教授级高级工程师。研究方向:药理毒理研究及中药新药开发电话:021-58958228StudyonPharmacodynamicsandtoxicoligyofLongkaiGranules(LKG)againstProstaticHyperplasiaXieJiajun1QianBaichu2GaoQi1ZhouGuangxing3CaiHuafang2QiaoZhengdong1ChengMiao1(1.ShanghaiInstituteofChineseMateriaMedica,Shanghai201203;2.InstituteofMateriaMedica,ZhejiangAcademyofMedicalSciences,Zhejiang,Hangzou310006;3.FudanUniversity,Shanghai200032)Keywords:LongkaiGranules(LKG);Prostatichyperplasia;Pharmacodynamics;Toxicology;Mice;Rats;dogsAbstract:Objective:TodemonstratetheinhibitoaryeffectsofLKCagainstexperimentalProstatichyperplasiaandevaluateitstoxicityonanimals第2页共6页第1页共6页编号:时间:2021年x月x日书山有路勤为径,学海无涯苦作舟页码:第2页共6页orally.Methods:Theprostateexponent,DNAcontentinprostatetissue、theactivityofacidphosphataseinserumorthewetweightofspermatophoresandtesticlesinnormalimmaturemiceandinthehyperplasiamodelmiceinducedbysubcutaneousinjectingtestoosleronespropionateorbyimplantingoftheurogenitalsinusweredeterminedafteradministratingofLKGintragastricallytothemice.ThesinglemaximumdosageofLKGinmiceanditslong-term(13weeks)toxicityinWistarratsandBeagledogsinorallywasevaluated.Results:LKGcandecreasetheweightsofprostatesandDNAcontentinthetissueinthenormalimmaturemiceafterbeingadministeredatdosageof20and40g/kgonceadays.LKG,atdosageofboth10,20and40g/kgfor10daysand20and40g/kgfor30days,caninhibitthehyperplasiaofventralprostatesinthemodelmiceinducedrespectivelybytheinjectionoftestoosleronespropionateandbyimplantingurogenitalsinus.LKG,atdosageof100g/kgfor13weekstoWistarrats,wouldleadtoprostaticatraphyinalightdegree,anditsepithelialcellschangeinshapefromcolumntoflatandprostaticcavitybeingsmall,whichdidnotrecoverin4weeksafterstopadministrationoftesteddrugtotheanimals.Thesinglemaximumdosagebyiginmiceis200g/kg.Therewasnosignificanttoxicityreactioninratsatdosageof10,40and100g/kgfor13weeksorinBeagledogsatdosageof12and60g/kgfor13weeks.Conclusion:LKCcaninhibittheprostatichyperplasiaandshowsnovisibletoxicityrea...