不同种类啤酒中氨基酸含量的分析[摘要]本论文是以啤酒为研究对象，探索不同种类啤酒中氨基酸的含量。本实验先对啤酒进行预处理，之后对预处理后的样品进行研究。啤酒中的氨基酸含量通过日立L-8900氨基酸全自动分析仪测定，该仪器灵敏、快速、提供数据准确可靠、分辨率高、操作简单、分析周期短。通过一系列实验之后，得出精酿啤酒比市售啤酒的总游离氨基酸含量高，且精酿啤酒与市售啤酒中的脯氨酸含量均为最高；而且精酿啤酒相较于市售啤酒口感更佳；两种啤酒中部分必需氨基酸高于FAO/WHO理想氨基酸构成比例，而部分则低于FAO/WHO理想氨基酸构成比例。[关键词]啤酒；氨基酸分析仪；游离态氨基酸IAnalysisofaminoacidcontentindifferentkindofbeerAppliedChemistryMajorjingruwang[Abstract]Inthispaper,thecontentofaminoacidsindifferentkindsofbeerwasstudied.Inthisexperiment,beerwaspretreatedandthenthetreatedsampleswerestudied.TheaminoacidcontentinbeerwasdeterminedbyHitachil-8900aminoacidautomaticanalyzer,whichissensitive,fast,accurateandreliable,highresolutionandeasytooperate.Afteraseriesofexperiments,itisfoundthatthecontentoftotalfreeaminoacidincraftbeerishigherthanthatincommercialbeer,andthecontentofprolineinbothcraftbeerandcommercialbeeristhehighest.Craftbeertastesbetterthancommercialbeer;SomeessentialaminoacidsinthetwobeerswerehigherthantheFAO/WHOidealaminoacidcompositionratio,whilesomewerelowerthantheFAO/WHOidealaminoacidcompositionratio.[Keywords]beer;aminoacidanalyzer;FreeaminoacidsII目录第一章绪论...................................................................................................................................................31.1啤酒的营养价值.......................................................................................................................................31.2本课题目前国内外研究情况..................................................................................................................31.3本课题研究意义及内容...........................................................................................................................4第二章实验部分...........................................................................................................................................62.1主要实验仪器..........................................................................................................................................62.2主要实验材料..........................................................................................................................................62.3啤酒的取样..............................................................................................................................................62.4实验方法及步骤......................................................................................................................................62.4.1氨基酸分析仪的操作原理............................................................................................................62.4.2氨基酸分析仪的色谱分析条件....................................................................................................72.4.3样品前处理....................................................................................................................................72.4.4上机................................................................................................................................................72.5结果与讨论......................
