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宁夏大学新华学院毕业论文（设计）（2013届）题目滩羊卵母细胞体外成熟体系建立专业生物技术年级2009学生学号12009248152学生姓名高欢指导教师李勇滩羊卵母细胞体外成熟体系建立摘要在细胞核移植过程中，体细胞必须在卵母细胞内进行重编程以恢复其全能性，卵母细胞成熟度直接影响体细胞重编程，故卵母细胞体外成熟培养是体细胞核移植极其重要环节，而卵母细胞又受环境，培养液浓度，培养温度等影响。本实验通过探讨不同浓度的FSH和LH及HMG影响滩羊卵母细胞体外成熟因素的研究，建立滩羊卵母细胞体外成熟体系，为滩羊核移植及以后的优良育种奠定基础。关键词：卵母细胞;FSH:LH;HMG;体外成熟；滩羊Reprogrammingofsomaticcelldirectly,sothatoocytesinvitromaturationplaysanextremelyimportomtroleinnucleartransplantationofsomaticcell.However,oocytesareaffectedbyenvironment,mediumconcentration,culturetemperatureandsoon;intheresearchwestudiedtheefforeofdifferentdensityFSHandLHandmixtureofFSHandLH,HMGdensity,onoocytesinvitromaturationintansheepandestablishedasystemofoocytesinvitromaturationintansheep,layingthefoundationforadvamcedbreedingandnucleartransplantationoftansheepKeywords:vitromaturation;FSH;LH;HMG;tansheep目录前言..................................................................................................................................................11实验材料与方法............................................................................................................................41.1实验材料.............................................................................................................................41.2主要仪器和耗材.................................................................................................................41.2.1主要仪器..................................................................................................................41.2.2试剂..........................................................................................................................41.3试验方法.............................................................................................................................51.3.1卵丘-卵母细胞复合体（cumulusoocytecomplexes,COCs）的收集：.............51.3.2卵丘-卵母细胞复合体的体外成熟培养：............................................................51.3.3卵母细胞体外成熟效果判断：.............................................................................62.实验分析.......................................................................................................................................62.1FSH和LH对滩羊卵母细胞体外成熟的影响..................................................................62.2HMG对卵母细胞体外成熟的影响...................................................................................83讨论与分析.................................................................................................................................104结论：..........................................................................................................................................11参考文献........................................................................................................................................12谢词................................................................................................................................................13前言滩羊是我国独特的裘皮用绵羊品种，是国家二级保护品种，2001年...