中国组织工程研究与临床康复第14卷第51期2010–12–17出版JournalofClinicalRehabilitativeTissueEngineeringResearchDecember17,2010Vol.14,No.51ISSN1673-8225CN21-1539/RCODEN:ZLKHAH95951FirstDepartmentofBoneSurgery,YejishanHospitalAffiliatedtoWannanMedicalCollege,Wuhu241001,AnhuiProvince,China;2CollegeofStomatology,AnhuiMedicalUniversity,Hefei230032,AnhuiProvince,ChinaLiQuan-hui★,Master,Attendingphysician,FirstDepartmentofBoneSurgery,YejishanHospitalAffiliatedtoWannanMedicalCollege,Wuhu241001,AnhuiProvince,Chinali2008_75@126.comCorrespondenceto:XuZhu-jun,Chiefphysician,CollegeofStomatology,AnhuiMedicalUniversity,Hefei230032,AnhuiProvince,ChinaSupportedby:theNaturalScienceFoundationofHigherEducationofAnhuiProvince,No.KJ2009A169*;theNationalNaturalScienceFoundationofChina,No.307724400*;ScienceandTechnologyResearchandDevelopmentProjectofAnhuiProvince,No.08010302196*Received:2010-07-01Accepted:2010-10-08胶原-肝素自组装多层膜修饰钛表面***★李全辉1，徐祝军2，李全利2，沈军2，张维波2Surfacemodificationoftitaniumbycollagenandheparinself-assemblymultilayersLiQuan-hui1,XuZhu-jun2,LiQuan-li2,ShenJun2,ZhangWei-bo2AbstractBACKGROUND:Thebiologicalsurfacemodificationforthetitaniumandimprovingthebiocompatibilityoftitaniumsurfaceisoneofthehotspotsinresearchoftitaniumsurfacemodification.OBJECTIVE:Collagenandheparinmultilayerswerecoatedonpuretitaniumusingalayer-by-layerself-assemblytechnique,toachieveextracellularmatrixmodificationofthetitaniumsurfaceandtoimprovethecytocompatibilityoftitanium.METHODS:Thefilmgrowthwasinitializedbydepositingonelayerofpositivelychargedpoly-L-lysineontheNaOH-treatedtitaniumsubstrate(porousandnegativelychargedsurface).Then,thefilmwasformedbythealternatedepositionofnegativelychargedheparinandpositivelychargedchitosanviaelectrostaticinteractionsofpolyelectrolytes,andterminatedwithanoutermostlayerofcollagen.Thechemicalcomposition,surfacetopographyaswellasroughnessofthemultilayerswereinvestigatedbyusingdiffusereflection-fouriertransforminfraredspectroscopy,scanningelectronmicroscope,atomicforcemicroscope,andcontactanglemeasurementrespectively.Weculturedbonemarrowstromalcellsonthesurfaceofmodified-titaniumandpuretitaniumrespectivelytoobserveitsadhesionandproliferation.RESULTSANDCONCLUSION:Theanalysisofdiffusereflection-fouriertransforminfraredspectroscopy,scanningelectronmicroscope,atomicforcemicroscope,andcontactanglemeasurementconfirmedthat,heparin-collagenmultilayersformedgraduallyonthetitaniumsurfaceandcouldimprovecelladhesion,proliferationanddifferentiation.Theheparin-collagenmultilayersisapotentialbiologicaltitaniumsurfacetoimprovethebiocompatibilityoftitaniumsurface.LiQH,XuZJ,LiQL,ShenJ,ZhangWB.Surfacemodificationoftitaniumbycollagenandheparinself-assemblymultilayers.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu.2010;14(51):9595-9600.[http://www.crter.cnhttp://en.zglckf.com]摘要背景：对钛表面进行生物化修饰，改善钛表面的生物相容性是目前钛表面改性研究的热点之一。目的：采用层层自组装技术，在钛表面构建胶原-肝素多层膜，实现钛表面的细胞外基质化修饰，以改善钛的细胞相容性。方法：首先采用NaOH处理钛基材，获得多孔、负电荷的钛表面；然后吸附1层正电荷的聚赖氨酸；最后，多次交替吸附负电荷的肝素和正电荷的胶原，形成以胶原为最外层的多层膜结构。通过漫反射红外光谱、扫描电镜、原子力显微镜、接触角测量对多层膜进行表征。并与骨髓基质细胞共培养，考察细胞的黏附、增殖情况。结果与结论：漫反射红...
