中南大学硕士学位论文白血病抑制因子及受体在多囊卵巢综合征中的表达姓名：潘琼申请学位级别：硕士专业：妇产科学指导教师：薛敏20050501中文摘要目的研究多囊卵巢综合征(PCOS)不孕患者血清白血病抑制因子(LIF)水平及卵巢组织中LIF和其受体(LIFR、gpl30)表达是否异常，从整体及细胞水平探讨卵巢局部调节因子LIF及其受体在PCOS排卵障碍中的作用。方法应用化学发光法及ELISA法测定22ffIIPCOS患者和20例输卵管性不孕患者血清性激素及血清LIF水平；应用免疫组织化学方法检测以上患者卵巢组织LIF及其受体的蛋白表达。结果1、PCOS组廊清中LIF的水平明显低于对照组(尸O．05)。3、LIFR在PCOS组颗粒细胞中呈阳性或强阳性表达，与对照组比较蠢漫著差吴(P>O。05)；闻覆细胞均璺弱阳性表达，比对照缝弱(P<0．05)；在卵泡膜细胞的表达两组无龆著差异(P>O．05)。4、gpl30禚两组颗粒细胞中的表达均弱，无盟著差异(P>0．05)，在卵泡膜及间矮细胞无表达。5、在两篷均可发现，LIF在颗粒纲腌中酶表达均是隧郭漉愈大纛愈强。LIFR在PCOS组颗粒细胞的表达无论卵泡大小均表现较强染色，未发现其染色随卵泡愈大简愈强的趋势；在对照组中，LIFR在颗粒细您的表达圈LIF类麓，照郛泡增大染惫增强。结论PCOS患者血清LIF水平的异常及LIF与其受体的调节失常可能是导致PCOS排卵障碍的一个黧要因素。关键词多囊卵巢综合征，岛盘病{；}每铡霭子，岛|薤病静裁霞子受体(LIFR)，gpl30ABSTRACTobjeetiveTodeterminewhethertheinfertilepatientswithpolycysticovariansyndromearerelatedtodyregulationofthelevelsofserumleukaemiainhibitoryfactor(LIF)andexpressionofLIFanditsreceptorsinovaries，andtocomparegeneralandlocalactionandtoexplainfurtherthefailureoffolliculardevelopmentregulatedbyovarianlocalfactors，suchasLIFanditsreceptorsinpolycysticovariansyndrome．Methods22infertilitypatientswithpolycysticovariansyndromeand20controlpatientswereincorporatedintothestudy．ThelevelsofserumLIFandgonadalhormonesweredetectedbyELISAandChemiluminescencerespectively．Expressionofleukaemiainhibitoryfactoranditsreceptorsweremeasuredbyimmunohistochemistry．Result1．Them．eanlevelofserumLIFofthestudygroupwasobviouslylowerthanthatofthecontrolgroup，P<0．05．andnegativelycorrelatedwit量lE2andL}薹，FSHratio．butcorrelatedwithoutPandT．2．StainingforLIFingranulosacellsofpolycysficovarieswassignificantlyweakerthanthatinthenormalovaries(P《O．001)．whereassimilarinthecaandintersititialcells(j》O．05)．3．StainingforLIFRininterstitialcellswassignificantlyweakerinpolycysticovariesthanthatinthenormalovaries(P0．05)．GranulosacellshadveryintensestainingforLIFR，butstainingforLIFRinthecacellswereveryweekinpolycysticovaries．霹．Intwogroups，thegranulosacellshadweakstainingforgpl30(P>O．05)，andinterstitialcellsandthecacellswerenegativeofimmunostainingforgpl30．5．Intwogroups，theintensityofimmunostainingforLIFobservedingranulosecellsincreasedasfolliclebecamelarge纛Inpolycysticovaries，theintensityofimmunostainingforLIFRingranulosacellslastedintensestronglyinatlsizeoffollicles，whichwasdifferentfromnormalovaries．Innormalovaries，theintensityofiImmmostainingforIIL1FRobservedingranulosecellsincreasedasfolliclebecamelargerConclusionKEYWORDSpolycysticovariansyndrome，leukaemiaInhibitoryfactor,LIFR，gpl30ln=炷竺：||差唧=湘积蛐羞原创性声明本人声明，所呈交的学位论文是本人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了论文中特别加以标注和致谢的地...
