生物工程下游技术-绪论VIP免费

DownstreamProcessingofBioengineering生物工程下游技术杨志红yangzhihong@hutc.zj.cn691612SectionOnePreface•Considerableeffortandtimeisallocatedtointroducingcellcultureandfermentationtechnologytoundergraduatestudentsinacademia,generallythrougharangeofcoursesinindustrialbiotechnologyandrelateddisciplines.Similarly,alargenumberoftextbooksareavailabletodescribetheapplicationsofthesetechnologiesinindustry.•However,therehasbeenagenerallackofappreciationofthesignificantdevelopmentsindownstreamprocessingandisolationtechnology,theneedforwhichislargelydrivenbythestringentregulatoryrequirementsforpurityandqualityofinjectablebiopharmaceuticals.Thisisparticularlyreflectedbythegeneralabsenceofcoverageofthissubjectinmanybiotechnologyandrelatedcoursesineducationalinstitutions.•ForaconsiderablewhileIhavefeltthatthereisincreasingneedforanintroductorytexttovariousaspectsofdownstreamprocessing,particularlywithrespecttotheneedsofthebiopharmaceuticalandbiotechnologyindustry.Althoughtherearenumeroustextsthatcovervariousaspectsofproteinpurificationtechniquesinisolation,thereisaneedforaworkthatcoversthebroadrangeofisolationtechnologyinanindustrialsetting.•ItisanticipatedthatDownstreamProcessingofProteins:MethodsandProtocolswillplayasmallpartinfillingthisgapandthusproveausefulcontributiontothefield.Itisalsodesignedtoencourageeducationalstrategiststobroadenthecoverageofthesetopicsinindustrialbiotechnologycoursesbyincludingaccountsofthisimportantandrapidlydevelopingelementoftheindustrialprocess.•Thehopeisthatthiswillresultingraduateshavingareasonableunderstandingofdownstreamprocessingprinciplesandtechniques,andthusbebetterpreparedtofulfilltheever-increasingdemandforcompetentisolationscientistsinindustries.•Thisis,ofcourse,achievedwiththehelpofthededicatedcontributingauthorsofDownstreamProcessingofProteins:MethodsandProtocols,withoutwhosewillingnesstocontributeandpatienceitwouldnothavebeenpossible.IwouldalsoliketothanktheHumanaPressandProf.JohnWalker(theserieseditor)fortheirencouragementandpromptfeedback.•MythanksarealsoduetotheMedevaPharmaDevelopmentmanagementforprovidingmewiththetimeandopportunitytofulfillthistask,andwithoutwhosesupportitwouldhavebeenimpossible.Finally,Iwishtothankmywife,children,andfamilymembersforallowingmetoperseverewithmyeditingactivitiesinperhapswhatshouldhavebeentheirtime.SectionTwo菌种筛选摇瓶实验发酵罐实验IndustrialScaleup•Totransferthepilotscaleresultsintoacommerciallyfeasibleproductionsetting.•Fermentorsizesrangefrom100Lto500,000L,dependingonproducts.在疾病治疗方面的应用生物制药抗生素基因治疗生物工程研究领域微生物培养动物细胞培养植物细胞培养天然资源生化材料海洋生物培养生物工程产业领域生物制药（抗生素，基因重组蛋白，氨基酸，疫苗，菌苗，天然药物，生化药物，血液制品，抗体，多糖，多肽）生物化工（乳酸，柠檬酸，苹果酸，丙烯酸，甘油，异丙醇，乙烯）生物能源（甲醇，乙醇，生物柴油，生物汽油）生物材料（明胶，胶原蛋白，人造皮肤，人造骨，人造脏器）生物医学（诊断试剂，基因治疗，人及生物克隆）环境生物（环境治理，水污染，土壤污染，风沙治理）生物食品（醋，啤酒业，酿酒，乳制品，奶制品）生物资源（动物，植物，微生物）生物农业（基因食品，基因植物）生物工程的上中下游上游：菌种，基因工程，分子生物学，遗传学中游：微生物发酵工程，动植物细胞，海洋生物培养下游：生物分离工程生物产品特点①产物浓度低的水溶液原因：a.氧传递限制；b.细胞量;c.产物抑制②组分复杂a....