益肾化湿微丸中防风、陈皮、清半夏的薄层鉴别目录1前言..............................................................12材料与仪器........................................................22.1实验材料........................................................22.2试剂和仪器......................................................23实验方法与结果分析................................................43.1防风薄层鉴别研究................................................43.2陈皮薄层鉴别研究...............................................143.3清半夏薄层鉴别研究.............................................214讨论.............................................................304.1防风...........................................................304.2陈皮...........................................................304.3清半夏.........................................................304.4展望...........................................................31参考文献...........................................................32综述.............................................................34致谢...............................................................40I益肾化湿微丸中防风、陈皮、清半夏的薄层鉴别摘要:目的本研究在益肾化湿原有的质量标准基础上，增加防风、陈皮和清半夏三味药材的薄层鉴别研究，提高产品标准，从而更好地控制产品质量和疗效，提高产品的市场竞争力；方法本实验在中国药典2015年版一部中防风、陈皮清半夏鉴别项下的基础上，通过优化方法和方法学验证，确定益肾化湿微丸中防风、陈皮和清半夏三味药的供试品制备方法和展开条件，并进行方法学考察，建立益肾化湿微丸中防风、陈皮和清半夏的薄层鉴别方法；结果药典中防风和陈皮的薄层鉴别方法都不适用于益肾化湿微丸，需要根据药物特点优化，药典中半夏的薄层鉴别方法（2）适用于鉴别益肾化湿微丸中的清半夏，即防风展开剂为二氯甲烷-甲醇-水（6.5:2.5:1），陈皮展开剂为三氯甲烷-甲醇-氨水-水（2:1:0.03:0.03），清半夏展开剂为正丁醇-冰醋酸-水（6:3:1），最终确定主斑点清晰、无干扰，重现性、专属性和耐用性都较好的TLC鉴别方法；结论三批中试样品验证，确定三味药材的TLC鉴别方法所对应的主斑点清晰、无干扰，该方法重现性、专属性和耐用性好，可作为益肾化湿微丸质量标准。关键词：益肾化湿微丸；防风；陈皮；清半夏；薄层鉴别注：本论文题目来源于教师的香港合作科研项目，项目编号为：UIM/290。IIIdentificationofthinlayerofDivaricateSaposhnioviaRoot,DriedTangerinePeelandPinelliainYishenHuashiPelletsAbstract:ObjectiveBasedontheoriginalqualitystandardofYishenHuashi,thisstudyincreasedthinlayeridentificationstudiesofDivaricateSaposhnioviaRoot,DriedTangerinePeel,andPinelliatrifoliatemedicinalherbstoimproveproductstandards,therebybettercontrollingproductqualityandcurativeeffect,andimprovingproductmarketcompetitiveness;MethodsThisexperimentwasbasedontheidentificationofDivaricateSaposhnioviaRoot,DriedTangerinePeell,andPinelliagenusintheChinesePharmacopoeia2015edition.Throughtheoptimizationmethodsandmethodvalidation,theexperimentwasconductedtodeterminetheDivaricateSaposhnioviaRoot,DriedTangerinePeel,andPinelliatrifoliiinYishenHuashiPills.Preparationmethodsanddeploymentconditionsforthetestsamples,andmethodologicalinvestigationswereconductedtoestablishathinlayeridentificationmethodforDivaricateSaposhnioviaRoot,DriedTangerinePeelandPinelliaternatainYishenHuashiPellets;ResultsThepharmacopoeiathinlayeridentificationmethodofDivaricateSaposhnioviaRootanddriedDriedTangerinePeeldoesnotapplytoYishenhuashiPelletsandneedstobeoptimizedaccordingtothech...
