常用缓冲溶液的配制方法1.H逋醸-盐輟缓冲液＜0,05IIH＞I/L＞X毫升0,2moIZLtt氨戦+Y竜升62nol/LHCL冉加水柿释竿200毫升PHXYPHXY2,05044.03,05011.42.45032.43.2508.22.6502d.23.-1506.42.85016.83,6505.0甘氨酸分子量-75,07.OJinol/L廿氨酸落液含仮D1畑升.2.绑苯1甲酸-盐酸缓冲液＜0朋moVUX左升0.2mol/L邻苯E卩酸轲钾-0^mol/LHC1冉J1水輛釋到pH(20€)XYpH(20t)XY2.254.0703a251.4702.4539603.450.9902,653.2953,650,5972.83.0552.6422.0223.850.263邻笨二甲醮氢钾分了虽=204.250.2uwl/L郭笨二甲酸星溶液40.85克/升N磷履氢：他-柠掾程缓冲總PHQ^mol/LNa2HPO4畅)0.1moLT.柠檬酸〔老升）PHO.ZIDHI/LNB2HPO4僱升)OJmol/L柠檬酸〔亳刃＞2.20.4010.605,210.729,282.41.2418.765.411.158.852,(52.1817.825.611..608.402.83.1716.835.812.097.913.04,1115.896.012.657.373.24.9415.066.213226.783.45.701-1306A13.856.15处6.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473534.28.2811.727.217.392.614.48.S211.1S7.4IS.171.S34.693510.657.618.73J.274.89.8610.147.SIS150.S55.010309.708.019.450.55M’HPOq分子呈=14.9ft0.2mm/L溶28.40加升■:Na2HPO4*21[2O7-量=178.050卫inoJ/L^^含35XH芯'斤：C4H2O7*比0分了吊=210.140.1iml/L潜液为21,01忠升*4.柠樣战-筑氧化钠-盐餓缓冲酒pH钠离f■浓度（mol/L）柠據酸（i）-H:O氢氧化钠（克）NaOH97%盐酸C湛升）HC'l（浓）彊终休积4.411.48.64.61039.7J.S9:210.SPH0.1m»l/L柠檬酸〔毫升｝0.1moVL柠樣酸钠〔至升）5.0_8.2「11.85.27.312,75.46.413.65,65.514,55.84.715.36.03.816.26.22.817.2&勻2.018.0651.418,6（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15moI/DpHmoVL/15Na2HPO4（毫升）mol/L//15KH2PO4（毫升）pHmoVL//15Na2H]（毫升）4.920.109.907.177.005.290.509.507.388.005.911.009.007.739.006.242.008.008.049.506.473.007.008.349.756.644.006.008.679.906.815.005.008.1810.006.986.004.00Na2HPO4-2H2O分子量=178.051/15M溶液为11.876克/升。KH2PO4分子量=136.0S1/15M溶液为9.078克/升。&磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2MKPO4+Y毫升0.2NNBOH加水稀释至29毫升pH（20°C）X壓升）Y（毫升）pH（20，＞C）x5.850.3727.056.050.5707.256.250.8607.456.451.2607.656.651.7807.856.852.3658.057.磷蟻盐缓冲液磷酸氢二钠-磷酸二氢钠缓冲蔽10,2）pH0.2mol/LNa2HPO4（毫升）0,3niol/L（毫心pH0.2LttdZLNa2HPO4（亳爪03mol/LNaH^POi5.8S.092.07.061.039,05.510.090.07.167.033.06.012387J7.272.02S.06.115.085.07.377.023.06218.5S1.57.481.015.06.322.577.57,584.016.06.426.573.57.687.013.06.531.568.57.789.510.56”637.56257.891.58,56543.556,5化993*07,06.849351.08.094.75.36£55,045,0Na2HPO^2H2O分丁母=178.0S0.2mol/L溶液沟孤紀M升■:Nd田POq-L2H2O^子量=35S.230.2UHJIZL溶破为了1.64克/升°Na」EP-：：■:'JI:'?7HI-rUPHmoJ：La5Na2HP04（毫升）mold'd5KH2PO4僵升）PH[nol/L/A5NaaHP01（毫升）...
