中药复方研究室细胞与分子实验操作指南（2009.9）目 录第一章 细胞实验操作·········································································3第一节 细胞培养常用耗材与设备························································3第二节 无菌操作·············································································3第三节 培养用品的清洗····································································5第四节 培养用品的消毒灭菌及灭菌锅的使用·········································6第五节 常用缓冲液、胰蛋白酶消化液及培养基的配制·····························8第六节 细胞培养、换液、消化传代···················································11第七节 细胞计数···········································································14第八节 细胞复苏与冻存··································································16第九节 MTT 法测定细胞活力或细胞增殖············································17第二章 分子实验操作········································································18第一节 PCR 引物设计与实验操作······················································18第二节 WESTERN BLOT 实验操作·······················································26第三节 FLOW CYTOMETRY 实验操作··...
