人牙周膜细胞在双氧水处理后纯钛表面的活性【关键词】 牙周膜； 细胞培养； 钛； 碱性磷酸酶； 人牙周膜细胞； 双氧水 ［Abstract］ Objective: To investigate the application possibility of hydrogen peroxide pre-treated titanium in oral clinic. Methods: The proliferation and alkaline phosphatase （ALP） activity of human periodontal ligament （hPDL） cells attached on titanium with four kinds of surface （smooth, rough, etched, hydrogen peroxide treated）were detected. The growth of primary and passaging cells was observed with invert microscope, and the morphology of cells was observed with scanning electronic microscope （SEM）; ALP activity was tested with enzyme-linked immunosorbant assay. The morphology, immunohistochemistry features, proliferation and ALP acyivity of cells on the four kinds of titanium were compared. Results: The hPDL cells could grow and proliferate on four kinds of titanium. The cell proliferation on titanium treated by hydrogen peroxide was better than those in the other three groups（P＜0.05）. Conclusion: Titanium treated by hydrogen peroxide is more beneficial to attachment and proliferation of the cells than titanium processed by the other three ways. Cells in the four groups all showed ALP activity, and among them, the activity in cells of hydrogen peroxide treated group is the highest（P＜0.05）. ［Key words］ periodontal ligament; cells culture; titantium; alkaline phosphatase; human periodontal ligament cells; hydrogen peroxide 种植体以其有效的支持、良好的咀嚼功能、方便的清洁和护理被广泛应用于口腔临床治疗中。理想的种植体要求种植体-软组织界面有类似天然牙上皮附着的结构，形成紧密的上皮“袖口”，成为一种功能性生物屏障。它有保护种植体在复杂的口腔环境中免受细菌及其他致炎因子侵入而保持长期稳定的作用。近代牙周病学已证明：牙周组织再生的细胞学基础是牙周膜细...
