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T e c h n i c a l B u l l e t i nCellTiter 96® AQueous OneSolution Cell ProliferationAssayINSTRUCTIONS FOR USE OF PRODUCTS G3580, G3581 AND G3582.PRINTED IN USA.Rev ised 6/09Part# TB245Promega Corporation·2800 Woods Hollow Road ·Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526· Phone 608-274-4330 · Fax 608-277-2516 · www.promega.comPrinted in USA.Part# TB245Revised 6/09Page 11. Description..........................................................................................................12. Product Components and Storage Conditions............................................43. Protocols..............................................................................................................5A. General Protocol...................................................................................................5B. Example of a Protocol for Bioassay of IL-6 Using B9 Cells...........................54. General Considerations....................................................................................6A. Background Absorbance.....................................................................................6B. Optional Wavelengths to Record Data.............................................................7C. Lymphocyte Assays.............................................................................................8D. Reagent Optimization..........................................................................................8E. Cell Number Optimization.................................................................................85. References...........................................................................................................96. Related Products................................................................................................91.DescriptionThe CellTiter 96®AQueousOne Solution Cell Proliferatio...