抗凝活性导向的三棱炮制工艺优化研究目 录摘要..............................................................................................................................ⅠⅡ1 前言.............................................................................................................................12 材料与仪器.................................................................................................................22.1 试剂与药材..............................................................................................................22.2 主要仪器..................................................................................................................23 试剂的前处理.............................................................................................................23.1 底物溶液的制备......................................................................................................23.2 凝血酶标准溶液的制备..........................................................................................24 实验方法.....................................................................................................................34.1 活性测定方法..........................................................................................................34.2 凝血酶初凝时间计算方法......................................................................................34.3 单因素实验设计......................................................................................................44.4 炮制工艺优化的响应面设计..................................................................................55 实验结果.....................................................................................................................55.1 单因素实验结果......................................................................................................55.2 响应面实验结果与分析........................................................................
