这个是急性单核白血病细胞，悬浮的（假如养的时间很长了有的会贴壁），培育基用普通的 1640 就可以了，这里是这个细胞株的详细资料供参考。THP-1： human acute monocytic leukemiaOrigin: established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse in 1978; the cells can be used for induction of differentiation studies; the cells were described to produce lysozyme and to be phagocytic; carries t(9;11)(p21;q23) leading to MLL-AF9 fusion geneMorphology: round, single cells in suspension, partly in clustersMedium: 90% RPMI 1640 + 10% FBSSubculture: maintain at 0.1-1.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days; seed out at ca. 0.5 x 106 cells/mlIncubation: at 37 °C with 5% CO2Doubling time: ca. 35-50 hoursHarvest: saturation density at about 1.0 x 106 cells/mlStorage: frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/mlMycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assaysImmunology: CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD33 (+), CD34 -, CD68 +, HLA-DR +Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profileSpecies: confirmed as human with IEF of AST, MDH, NPCytogenetics: human near-tetraploid karyotype - 94(88-96)<4n>XY/##Y, -Y, +1, +3, +6, +6, -8, -13, -19, -22, -22, +2mar, add(1)(p11), del(1)(q42.2), i(2q), del(p21)x2-4, i(7p), der(9)t(9;11)(p22;q23)i(9)(p10)x2, der(11)t(9;11)(p22;q23)x2, add(12)(q24)x1-2, der(13)t(8;13)(p11;p12), add(?18)(q21) - carries t(9;11) associated with AML M5Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II - THP-1 单核细胞是悬浮生长的细胞，用 1640＋10％FBS 是很容易养的，但复的细胞可适当加量至 20%FBS。...
