122中国临床康复第巷第，9朋2006-05-20出版ChineseJou．mlofClinicalRehabilitation,May202006VoL10No．19电针胃经穴后胃溃疡大鼠胃黏膜细胞蛋白激酶C活性的变化严洁，杨宗保，邹晓平，易受乡，常小荣，林亚萍·基础研究·湖南中医药大学针灸推拿学院针灸经络研究所。湖南省长沙市41O007严洁，女，l941年生，湖北省武汉市人，汉族，1963年武汉中医学院毕业，教授，博士生导师。主要从事经脉脏腑相关规律厦机制的研究。国家自然科学基金重大研究项目(90209023)中田分类号：R573文献标识码：A文章编号：1671-5926(2006)19-0122-03收稿日期：2006-03一o7修回日期：2006—04—19(o6—50—3一l870／Y·LL)ChangesofproteinkinaseCexpressioningastricmucouscellsofratswinlgastriculcerfollowingelectroacupunctureatgastricmeridianacupointYanJieYangZong—bao，ZouXiao—ping，YiShou-xiang，ChangXJao—rang，LinYa-pingInstituteofAcupunctureandMeridians，DepartmentofAcupunctureandMoxibustion．HunanCollegeofTraditionalChineseMedieine，Changsha4looo7．HunanProvince．ChinaYanJie．Professor，Tlut0rofdoctor，InstituteofAcupunctureandMeridians，DepartmentofAcupunctureandMoxibustion。HunanCollegeofTraditionalChineseMedicine．Changsha4lO0O7，HunanProvince．ChinaSupportedby：theMajorResearchProgramofNationalNaturalScienceFoundationofChina，No．90209023*Received：2006—03—07Accepted：2006-O4一l9Abstract删：ToobservetheefectofelectroaeupuneturegastricmeridianonproteinkinaseCfPKC1expressioningastricmucouseellofratswithstressulcerofstomach，MIETHODS：TheexperimentwasconductedattheAeupunetureandMoxibustionlaboratoryofHunanCollegeofTraditiona1ChineseMedicinefromJulytoDecember2005．SixtyhealthySDratswererandomlydividedintoulcerserum(US)group，gastricmeridiansen／m(GMS)group(acupoints：sibai，liangmen，zusanli)，gallbladdermeridianserunl(GBMS)group(acupoints：yangba／，riyue，yanglingquan)，GMS+PDl53o35groupandGBMS+PDl53O35groupwith12ratsineachgroup．Thegastriculcermodelswereestablishedbywater-immersionandrestraintmethod．Themedicaleleetroaeupuncturestimulator(ModelG6805一l1wasusedfor30minuteswith4Hzintermittentwave。20Hzirregularwave，andtheintensityw聃limitedtothelightvibrationofmuscleortheneedleholder．Th。gastricmucouscellwereseparatedbypmnasedigestionmethodandincubatedbyepidermalgrowthfactorreceptorinhibitorPD153035andserum．ThePKCexpressionongastricmucouseellwasdetectedbyisotopeminglemethod，RESULTS：AUthe60ratswereinvolvedintheresultanalysis．TherewaslittlePKCexpressionintheUSgroup[(26．16~30．94)nkat／g]；whileithadaimprovedtendencyintheGMSandGBMSgroups，especiallytheGMSgroup，andthereweresignificantdiferencesbetweenthetwogroups[(114．92~65．61)，(36．47~27，54)nkat／g,P<0．01】；littlePKCexpressionswerefoundintheGMS+PDl53035andGBMS+PDl53035groups．whichhadsignificantdifferences【(17．39~23．39)，(23．44±l4．42)nkat／g，P<0．011．CONCLUSIoN：TheacupuncturecanstimulatetheactivationofPKCingastricmucouseellsignalconductivepathwayofrats，andthereisaspecialcorelationbetweenmeridianandinternalorgansYanJ,YOngZB，ZhouXP，YiSX,ChangXR，LinYPChongesofproteinkinaseCexpressioningastricmucouscellsofrutswithgostriculcerfoIlowingeledroocupundureelgostricmeridionacupoint,ZhongguoLinchuartg∽2006；10(191：122—4(ChinaJ严洁，杨宗保，邹晓平，易受乡，常／J＼秉，林亚萍电针胃经穴后胃溃疡大鼠胃黏膜细胞蛋白激酶C活性的变化uIl中国临床康复，2006。10(19)：122—41wwwzglck'fcoml摘要目的：观察电针胃经(穴)对应激性胃溃疡大鼠胃黏膜细胞蛋白激酶C活性的影响。方法：实验于2005—07／1...
