Astandardizedmethodtocreateopticnervecrush:Yasargilaneurysmclip建立视神经挤压伤的标准方法:Yasargil(脑)动脉瘤夹LeventSarikcioglua,*,NecdetDemirb,c,ArifeDemirtopcaDepartmentofAnatomy,AkdenizUniversity,FacultyofMedicine,07070Antalya,Turkeya土耳其,07070Antalya,Akdeniz大学医学院,解剖部bDepartmentofHistologyandEmbryology,AkdenizUniversity,FacultyofMedicine,07070Antalya,Turkeyb土耳其,07070Antalya,Akdeniz大学医学院,组胚部cElectronMicroscopyUnit(TEMGA),AkdenizUniversity,FacultyofMedicine,07070Antalya,Turkeyc土耳其,07070Antalya,Akdeniz大学医学院,电镜部Received4August2006;acceptedinrevisedform18October2006Availableonline8December20062006-8-4收稿；2006-10-18校后发表；2006-12-8在线发表AbstractItisoftendifficulttocompareresultsobtainedbydifferentinvestigatorsonnervecompressioninjuries,owingtodifferencesinmethodofpressureapplicationandnoncomparablepressurelevels.Inthepresentstudy,wedescribedanewmethodtocrushtheopticnervebyusingaspeciallydesignedandcommerciallyavailabledevice.Wethinkthatstandardizationofthecompressionmethodsisnecessarytocompareinterlaboratoryresults._2006ElsevierLtd.Allrightsreserved.摘要：由于不同研究者建立神经挤压伤过程中使用方法不同,压力程度不同,通常要比较所得出的结果很困难。现在，描述一种新的建立神经挤压伤的方法，此方法采用的设备专门设计且市面有售。我们认为，为了比较各项试验结果，非常有必要将挤压伤的方法标准化。Keywords:Yasargilaneurysmclip;crushinjury;opticnerve关键词：Yasargil（脑）动脉瘤夹；挤压伤；视神经1.Introduction1．引言Theopticnerveoftheratisaveryvulnerablestructure(Gellrichetal.,2002).Isolatedpositionoftheopticnerveallowsthescientisttocreatelesionsconveniently,withoutdamagingotherpartsofthebrain.Anotheradvantageoftheopticnervelesionmodelisthatrecoveryofthefunctioncanbestudiedinawell-definedanatomicalsystemwithspecifictestsofvisualbehavior(SabelandAschoff,1993).鼠视神经结构非常易损伤（Gellrich等，2002）。视神经位置孤立，这使科学家很方便建立损伤，不会损坏脑的其它部分。视神经损害模型另一优势在于可以在确定的解剖系统中通过针对视觉反应的特定试验研究其功能恢复情况（SabelandAschoff,1993）。Traumatotheopticnervecausedbyeitherfracturesofthemidfaceand/orskullbasehasbeensimulatedbyopticnervecrushinjurymodel(Gellrichetal.,2002).Themaindrawbackofmostofthemethodsdescribedforopticnervecrushisthattheinjurycannotbequantifiedatthesiteofthetrauma,butisrathersemiquantitativelyreferredtoaspressureinaballoon(Burkeetal.,1985,1986;Cotteeetal.,1991),distancebetweenthetipsofaforceps(Buysetal.,1995;Duvdevanietal.,1990;SautterandSabel,1993),orpullingforceonamicro-sling(Matsuzakietal.,1982).Gellrich等人已通过制作视神经挤压伤模型模拟面中部骨折和/或颅底骨折所致视神经损伤（Gellrich等，2002）。以往所述大部分制作视神经挤压伤模型方法的主要缺点在于创伤位置的损伤不能定量，而是参考气舱中压力（Burke等，1985，1986；Cottee等，1991）、镊子尖端之间距离（Buys等，1995；Duvdevani等，1990；Sautter和Sabel，1993）、或微悬带上拉力（Matsuzaki等，1982）半定量。Intheliterature,thereareseveralmethodstocreateopticnervecrushinjury.However,themaindisadvantagesofthesemethodsarestandardizationandtheirpossibleusagebyinvestigatorsfromallovertheworld.Inourpreviousstudywedescribedasimpleandreliabledevice,theYasargilaneurysmclip,tocreatesciaticnerveinjury.Inthiscurrentstudy,ourgoalwastodescribeasimplemethodthatsimulatestraumatotheopticnervecausedbyfracturesofthemidfaceand/orskullbase,inordertoinvestigatetheeffectsofopt...
