活血祛瘀方对单侧输尿管梗阻大鼠肾间质细胞外基质的调节沈烨渠1，2，周健淞3，沈丽萍4，何立群1，5(1.上海中医药大学附属曙光医院肾内科，上海200021;2.肝肾疾病病证教育部重点实验室上海中医药大学，上海201203;3.南通大学第三附属医院肾内科，江苏无锡214000;4.上海市杨浦区中医医院内科，上海200041;5.上海市高校中医内科E－研究院上海中医药大学，上海201203)摘要:目的:通过研究中药复方活血祛瘀方对单侧输尿管梗阻(UUO)大鼠肾组织α－平滑肌肌动蛋白(α－SMA)、纤维连接蛋白(FN)、纤溶酶原激活物抑制剂－1(PAI－1)及肝细胞生长因子(HGF)表达的影响，探讨其对细胞外基质(ECM)的调节机制。方法:建立大鼠UUO模型，48只SD大鼠随机分为假手术组、模型组、代文组、活血祛瘀方组，于治疗4周后取梗阻侧肾组织，免疫组织化学染色观察α－SMA、FN、PAI－1及HGF的阳性染色表达，并进行半定量分析。结果:与假手术组相比，模型组大鼠α－SMA、FN、PAI－1阳性表达显著升高(P＜0.01)。与模型组相比，活血祛瘀方组肾间质α－SMA、FN及PAI－1阳性表达显著下调(P＜0.01)，而HGF阳性表达则显著上调(P＜0.01)。结论:活血祛瘀方可抑制肾组织α－SMA、FN及PAI－1过度表达，诱导HGF高表达，从而减少ECM的过度聚集，这可能是活血祛瘀方抗肾间质纤维化的作用机制之一。关键词:活血祛瘀方;单侧输尿管梗阻;细胞外基质;肾间质纤维化;机制中图分类号:R285.5文献标识码:A文章编号:1000－1719(2010)09－1831－04Regulationof“HuoxueQuyurecip”onExtracellularMatrixinRatsafterUnilateralUreteralObstructionSHENYe-qu1，2，ZHOUJian-song3，SHENLi-ping4，HELi-qun1，5(1.NephrologyDepartmentofShuguangHospitalAffiliatedtoShanghaiUniversityofTraditionalChineseMedicine，Shanghai200021，China;2.KeyLaboratoryofLiverandKidneyDiseases(ShanghaiUniversityofTraditionalChineseMedicine)，MinistryofEducation，Shanghai201203，China;3.NephrologyDepartmentoftheThirdHospitalAffiliatedtoNantongUniversity，Wuxi214000，Jiangsu，China;4.MedicalDepartmentofTraditionalChineseMedicineHospitalofShanghaiYangpuDistrict，Shanghai200041，China;5.TraditionalChineseInternalMedicineE－instituteofShanghaiUniversity(ShanghaiTCMUniversity)，Shanghai201203，China)Abstract:Objective:Toinvestigatetheeffectsof“HuoxueQuyurecip”，acompoundtraditionalChineseherbalmedicine，onexpressionofalpha－smoothmuscleactin(α－SMA)，fibronectin(FN)、plasminogenactivatorinhibitor－1(PAI－1)andhepa-tocytegrowthfactor(HGF)inrenaltissueofratswithunilateralureteralobstruction(UUO)andtoilluminatetheregulationmecha-nismsofextracellularmatrix(ECM)．Methods:48Sprague－Dawleyratswereunilaterallyobstructedbyligationoftheproximalu-retersandrandomlydividedintosham－operatedgroup，modelgroup，Diovantreatedgroup，and“HuoxueQuyurecip”treatedgroups．Theratsweresacrificedonthe4thweekaftertreatment，andimmunohistochemistrystainingforsemiquantitativeanalysisofα－SMA、FN、PAI－1andHGF．Results:Theleverofα－SMA、FNandPAI－1inmodelgroupincreasedascomparedwiththoseinthesham－operatedgroup(P＜0.01)。Incomparisonwiththemodelgroup，theexpressionofpositivestainingforα－SMA、FNandPAI－1in“HuoxueQuyurecip”treatedgroupweresignificantlydown－regulated(P＜0.01);buttheexpressionofHGFweresignificantlyup－regulated(P＜0.01)．Conclusion:“HuoxueQuyurecip”coulddecreasetheexcessiveaggregationofECMviainhibitingtheproteinexpressionsofα－SMA、FNandPAI－1，inducingtheexpressionofHGF，whichmayexplainoneofthemechanismsofitsantifibroticeffect．Keywords:HuoxueQuyurecip;UnilateralUreteralObstruction;ExtracellularMatrix;RenalInterstitiumFibrosis;Mecha-nism．收稿日期:2010－01－27基金项目:国家自然科学基金项目(...
