辽宁中医杂志2010年第37卷第9期·183l·活血祛瘀方对单侧输尿管梗阻大鼠肾间质细胞外基质的调节沈烨渠。周健淞，沈丽萍。何立群(1．上海中医药大学附属曙光医院肾内科，上海200021；2．肝肾疾病病证教育部重点实验室上海中医药大学，上海201203；3．南通大学第三附属医院肾内科，江苏无锡214000；4．上海市杨浦区中医医院内科，上海200041；5．上海市高校中医内科E一研究院上海中医药大学，上海201203)摘要：目的：通过研究中药复方活血祛瘀方对单侧输尿管梗阻(UUO)大鼠肾组织一平滑肌肌动蛋白(o【一SMA)、纤维连接蛋白(FN)、纤溶酶原激活物抑制剂一1(PAl一1)及肝细胞生长因子(HGF)表达的影响，探讨其对细胞外基质(ECM)的调节机制。方法：建立大鼠UUO模型，48只SD大鼠随机分为假手术组、模型组、代文组、活血祛瘀方组，于治疗4周后取梗阻侧肾组织，免疫组织化学染色观察—SMA、FN、PAI一1及HGF的阳性染色表达，并进行半定量分析。结果：与假手术组相比，模型组大鼠ot—SMA、FN、PAl—l阳性表达显著升高(P<0．01)。与模型组相比，活血祛瘀方组肾间质ot—SMA、FN及PAl一1阳性表达显著下调(P<0．01)，而HGF阳性表达则显著上调(P<0．01)。结论：活血祛瘀方可抑制肾组织d—SMA、FN及PAl一1过度表达，诱导HGF高表达，从而减少ECM的过度聚集，这可能是活血祛瘀方抗肾间质纤维化的作用机制之一。关键词：活血祛瘀方；单侧输尿管梗阻；细胞外基质；肾间质纤维化；机制中图分类号：R285．5文献标识码：A文章编号：1000—1719(2010)09—1831一o4Regulationof‘‘HuoxueQuyurecip”onExtraceHularMatrixinRatsafterUnilateralUreteralObstructionSHENYequ’，ZHOUJian—song，SHENLi-ping"，HELi—qun’’(1．NephrologyDepartmentofShuguangHospitalAfiliatedtoShanghaiUniversityofTraditionalChineseMedicine，Shanghai200021，China；2．KeyLaboratoryofLiverandKidneyDiseases(ShanghaiUniversityofTraditionalChineseMedicine)，MinistryofEducation，Shanghai201203，China；3．NephrologyDepartmentoftheThirdHospitalAfiliatedtoNamongUniversity，214000，fiangsu，China；4．MedicalDepartmentofTraditionalChineseMedwineHospitalofShanghaiYangpuDistrict，Shanghai200041，China；5．TraditionalChineseInternalMedicineE—instituteofShanghaiUniversity(ShanghaiTCMUniversity)，Shanghai201203，China)Abstract：0ctive：Toinvestigatetheefectsof“HuoxueQuyurecip”，acompoundtraditionalChineseherbalmedicine，onexpressionofalpha—smoothmuscleactin(—SMA)，fibronectin(FN)、plasminogenactivatorinhibitor一1(P^J一1)andhepa—tocytegrowthfactor(HGF)inrenaltissueofratswithunilateralureteralobstruction(UUO)andtoilluminatetheregulationmecha—nismsofextraeellularmatrix(ECM)．Methods：48Sprague—Dawleyratswereunilaterallyobstructedbyligationoftheproximalu—retersandrandomlydividedintosham—operatedgroup，modelgroup，Diovantreatedgroup，and“HuoxueQuyurecip”treatedgroups．Theratsweresacrificedonthe4thweekaftertreatment，andimmunohistochemistrystainingforsemiquantitativeanalysisof0L—SMA、FN、PAl一1andHGF．Results：Theleverofd—SMA、FNandPAI一1inmodelgroupincreasedascomparedwiththoseinthesham—operatedgroup(P<0．O1)。Incomparisonwiththemodelgroup，theexpressionofpositivestainingfor—SMA、FNandPAl一1in“HuoxueQuyurecip”treatedgroupweresignificantlydown—regulated(P<0．01)；buttheexpressionofHGFweresignificantlyup—regulated(P<0．O1)．Conclusion：“HuoxueQuyurecip”coulddecreasetheexcessiveaggregationofECMviainhibitingtheproteinexpressionsof一SMA、FNandPAl一1，inducingtheexpressionofHGF，whichmayexplainoneofthemechanismsofitsantifibroticefect．Keywords：HuoxueQuyurecip；UnilateralUreteralObstruction；ExtracelularMatrix；RenalInterstitiumFibrosis；Mecha—nism．收...
