世界中西医结合杂志2014年第9卷第4期WorldJournalofIntegratedTraditionalandWesternMedicine2014，Vol.9，No.4·实验研究·基金项目:深圳市龙岗区科技发展基金项目(No．YS2010007)作者单位:深圳市龙岗区第二人民医院，广东深圳518112通讯作者:李晓峰，Email:lxf19630505@126．com六味地黄丸对糖尿病伴牙周炎大鼠牙周组织中OPG与ＲANKL的影响李晓峰郭丽云张孝华刘建华陈伟程【摘要】目的通过研究六味地黄丸对糖尿病伴牙周炎大鼠牙周组织中骨保护素(OPG)与核因子－κB受体活化子配体(ＲANKL)的影响，探索六味地黄丸对牙周炎治疗的作用机制。方法选用清洁级6周龄雄性SD大鼠30只，采用腹腔注射链脲佐菌素(STZ)诱导大鼠糖尿病模型，采用钢丝结扎法建立牙周炎模型。动物模型建立后，随机分成实验组和对照组，实验组进行六味地黄丸灌胃，对照组采用蒸馏水灌胃;并于灌药后8周处死动物，解剖上颌骨，制作3μm厚连续切片，荧光免疫组化检测牙周组织中ＲANKL、OPG表达。激光共聚焦显微镜(LSCM)采集图像，行灰度值分析，比较OPG和ＲANKL在糖尿病实验组和对照组大鼠牙周组织中的表达差异。结果灌药后8周，实验组的OPG灰度值比对照组增加，且差异有统计学意义(P＜0．05)，而实验组ＲANKL灰度值比对照组明显降低，差异有统计学意义(P＜0．05)。结论六味地黄丸对糖尿病伴牙周炎大鼠的牙周组织炎症有明显抑制作用，六味地黄丸对牙周炎治疗机制可能是通过ＲANKL－细胞核因子κB受体活化因子(ＲANK)－OPG系统来实现。【关键词】六味地黄丸;牙周炎;糖尿病;核因子－κB受体活化子配体;骨保护素【中图分类号】Ｒ285．5;Ｒ587．2【文献标识码】A【文章编号】1673－6613(2014)04－0354－03ImpactsofLiuweiDihuangPillsonOPGandＲANKLinPeriodontalTis-sueoftheＲatswithDiabetesComplicatedwithPeriodontitisLIXiao－feng，ZHANGXiao－hua，LIUJian－hua，GUOLi－yun，CHENWei－cheng(DepartmentofStomatology，ShenzhenLonggangDistrictSecondPeople'sHospital，ShenzhenGuangdong518112)【Abstract】ObjectiveTostudytheimpactsofLiuweiDihuangPillsonOPG(osteoprotegerin)andＲANKL(receptoractivatorofnuclearfactor－κBligand)inperiodontaltissueoftheratswithdiabetescom-plicatedwithperiodontitissoastoexploretheeffectivemechanismofLiuweiDihuangPillsonperiodontitis．MethodsThirtyclean－grade，maleSDratsof6weeksoldwerecollected．TheintraperitonealinjectionofSTZ(streptozotocin)wasusedtoinducediabetesmodelsintherats．Theligationwithsteelwirewasappliedtosetupperiodontitismodel．Aftermodeling，theanimalmodelswererandomizedintoanexperimentalgroupandacontrolgroup．Intheexperimentalgroup，thegastricinfusionofLiuweiDihuangWanwasused．Inthecon-trolgroup，thegastricinfusionofdistilledwaterwasused．Theanimalsweresacrificedin8weeksafterinfu-sion．Themaxillarybonewasdissectedandpreparedcontinuouslyastheslices3μminthickness．Fluores-cenceimmunohistochemicaldetectionwasadoptedtodeterminetheexpressionsofＲANKLandOPGinperio-dontaltissue．Thelaserscanningconfocalmicroscope(LSCM)wasusedforimagecollectionandgreylevela-nalysis．ThedifferencesinＲANKLandOPGexpressionsinperiodontaltissuewerecomparedbetweentheex-perimentalgroupandthecontrolgroup．ＲesultsIn8weeksaftergastricinfusion，OPGgreyvalueintheex-perimentalgroupwasincreasedascomparedwiththecontrolgroup，indicatingthesignificantdifference(P＜0．05)．ＲANKLgreyvalueintheexperimentalgroupwasreducedapparentlyascomparedwiththecontrolgroup，indicatingthesignificantdifference(P＜0．05)．ConclusionLiuweiDihuangPillshastheobvioussuppressiononperiodontalinflammatorysymptomintheratsofdiabetescomplicatedwithperiodontitis．TheeffectivemechanismisachievedprobablyviaＲANKL－ＲNK－OPGsystem．【Keywords】LiuweiDihuangPills;Periodontitis;D...
