荞麦内转录间隔区(ITS)的扩增及序列分析-四川农业大学VIP免费

荞麦内转录间隔区（ITS）的扩增及序列分析生物科学 2002 级 曾子贤指导老师 吴 琦 副教授摘 要：本研究以野生金荞麦、栽培苦荞为材料，采用改进的 CTAB 法提取荞麦总DNA。设计一对特异性引物，对三份荞麦 ITS 区序列进行 PCR 扩增，均获得长约 700bp的单一条带。PCR 产物测序结果表明，野生金荞麦 1 号测序片段为 699bp，包含 ITS 区序列为 660bp；野生金荞麦 2 号测序片段为 678bp，包含 ITS 序列为 646bp；苦荞测序片段为640bp，包括 ITS 序列为 599bp。将所得到的三份荞麦 ITS 区序列分别与 GenBank 中登录的荞麦 ITS 序列进行比较。结果表明，野生金荞麦 1 号与已知金荞麦 ITS 区同源率为90.8%，野生金荞麦 2 号与已知的金荞麦 ITS 区序列同源率达到 91.6%。供试苦荞与已知苦荞 ITS 区同源率为 99.8%。关键词：野生金荞麦；苦荞；内转录间隔区（ITS）序列；5.8S rDNA；PCR；序列分析Amplification and Sequences analysis of Internal Transcribed Spacer of BuckwheatZENG Zi-xian Biological Science, Grade 2002 Directed by WU Qi (Associate Prof. Ph. D)Abstract：Wildness F. cymosum and cultivated F. tataricum were investigated in this article. By the improving method of CTAB DNA extraction, total DNA from three buckwheat was obtained. A pair of specific primers was designed. The ITS regions of three buckwheat were amplified. The amplified fragments, about 700bp in length, were sequenced directly. The results show that the sequencing fragments are 699bp, 678bp and 640bp in length, including ITS regions of F. cymosum and F. tataricum are 660bp, 646bp and 599bp in length. Comparing F. cymosum and F. tataricum with sequences reported, it suggests that the homology rate of ITS sequence of F. cymosum, NO. 1 is 91.6% and NO. 2 is 90.8% and the homology rate of the ITS sequence of F. tararicum is 99.8%.Keywords: Wildness F. cymosum; F. tataricum; ITS sequences; 5.8S rDNA; Sequence Analysis荞麦属于蓼科（Polygonaceae）荞麦属（Fagopyrum Mil...