利用PMSG和LRH-A3诱导间情期秋田犬发情效果的研究摘要为了探索提高母犬利用年限和终身产仔数。以不同剂量和给药次数的PMSG与LRH-A3组合的方法，对乏情期母犬进行外源激素诱导发情。第一组为皮下注射PMSG1500IU∕条，仅注射一次；第二组为皮下注射PMSG500IU∕条，少量多次，总剂量1500IU。当两组中的母犬综合征状显示最佳配种时同时注射一次LRH-A325ug∕条；第三组为空白对照组不注射任何药物，为母犬正常状态。结果，第二组采用少量多次皮下注射PMSG（500IU）进行诱导发情的发情率为37.5%，比第一组一次注射PMSG(1500IU)的发情率75%，高37.5%（P<0.05）。而第一组的受胎率为33.33%，第二组的受胎率是50%，两者相差16.67%（P<0.05）。试验证明，在外源性激素PMSG诱导母犬发情排卵时，在配种的同时应用LRH-A3可以促进卵泡发育和排卵，增加可用胚胎数和提高受胎率。关键词：孕马血清,促性腺激素释放激素，秋田犬，诱导发情UtilizationofestrusEstrusAkitabetweenPMSGandLRH-A3inducedAbstractInordertoexploretheuseofageandimprovelifelongbitcheslittersize.InPMSGandmethodsLRH-A3combinationsofdifferentdosageandadministrationtimesofanestrusbitchesexogenoushormonesinduceestrus.ThefirstgroupwasinjectedsubcutaneouslyPMSG1500IU/bar,injectedonlyonce;thesecondgroupwasinjectedsubcutaneouslyPMSG500IU/bar,afewtimes,atotaldoseof1500IU.Whentwoofthebestbreedingbitchsyndrome-likedisplaysimultaneouslyinjectedonceLRH-A325ug/bar;thethirdgroupwasthecontrolgroupwerenotinjectedanydrugforbitchesnormalstate.Asaresult,thesecondgroupreceivedasmallamountofmultiplesubcutaneousPMSG(500IU)forinductionofestrusestruswas37.5%,comparedwiththefirstgroup,thefirstinjectionofPMSG(1500IU)75%ofestrus,high37.5%(P<0.05).Thefirstgrouppregnancyratewas33.33%,thesecondgrouppregnancyrateis50%,adifferenceof16.67%(P<0.05).TestsshowthatexogenoushormonePMSGinducingovulationduringestrusbitchesinbreedingatthesametimeapplyLRH-A3canpromotefolliculardevelopmentandovulation,increasethenumberofavailableembryosandincreaseconceptionrate.Keywords:pregnantmareserumgonadotropin-releasinghormone,Akitas,induceestrus目录前言...............................................................11材料与方法........................................................21.1材料........................................................21.1.1试验时间和地点.........................................21.1.2试验动物...............................................21.1.3试验仪器与药品.........................................21.2方法........................................................21.2.1试验犬的饲养管理.......................................21.2.2激素处理方案及妊娠确定条件.............................21.2.3外部观察法、试情法及最佳配种时间确定...................31.2.4阴道涂片镜检及最佳配种时间确定.........................32结果与分析........................................................42.1注射一次PMSG母犬发情状况结果...............................42.2多次皮下注射PMSG诱导母犬发情状况结果.......................52.3采用PMSG不同注射方法和剂量诱导母犬发情状况结果.............63讨论..............................................................63.1犬发情不稳定、不明显、配不上原因分析........................63.2外源性激素在体内转化作用机理................................73.3处理方法的分析..............................................84结论..............................................................8参考文献............................................................9致谢.............................................................111前言随着...
