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中药核桃青皮提取物对食管癌细胞增殖的抑制作用及其分子机制的探讨的开题报告

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精品文档---下载后可任意编辑中药核桃青皮提取物对食管癌细胞增殖的抑制作用及其分子机制的探讨的开题报告摘要:食管癌是一种恶性肿瘤,其发生和进展与多种因素相关。中药被广泛地应用于食管癌的治疗和预防,其中核桃青皮被认为具有一定的抑制食管癌细胞增殖的作用。本文旨在通过讨论中药核桃青皮提取物对食管癌细胞增殖的抑制作用及其分子机制,为其临床治疗提供依据。本讨论将采纳 CCK-8 法测定核桃青皮提取物不同浓度对食管癌细胞(Eca109)增殖的影响,并通过流式细胞术和 Western blot 分析核桃青皮提取物诱导食管癌细胞凋亡和细胞周期的变化。本文还将采纳实时定量 PCR 技术检测核桃青皮提取物对食管癌细胞相关基因的表达水平的影响。估计结果中药核桃青皮提取物能够显著地抑制食管癌细胞(Eca109)的增殖,并能够诱导食管癌细胞凋亡和细胞周期的变化。此外,核桃青皮提取物还将影响食管癌细胞相关基因的表达水平。关键词:中药;核桃青皮;食管癌;细胞增殖;分子机制。Abstract:Esophageal cancer is a malignant tumor, its occurrence and development are related to many factors. Chinese medicine has been widely used in the treatment and prevention of esophageal cancer. Among them, walnut green peel is considered to have a certain inhibitory effect on the proliferation of esophageal cancer cells. This study aimed to explore the inhibitory effect of Chinese herbal medicine walnut green peel extract on esophageal cancer cell proliferation and its molecular mechanism to provide a basis for its clinical treatment.In this study, the CCK-8 method will be used to determine the effect of different concentrations of walnut green peel extract on the proliferation of esophageal cancer cells (Eca109), and the changes in apoptosis and cell cycle of esophageal cancer cells induced by walnut green peel extract will be analyzed by flow cytometry and Western blot. Real-time quantitative PCR technology will also be used to detect the 精品文档---下载后可任意编辑effect of walnut green peel extract on the expression levels of esophageal cancer cell-related genes.It is expected that Chinese herbal medicine walnut green peel extract can significantly inhibit the proliferation of esophageal cancer cells (Eca109), induce apoptosis and cell cycle changes of esophageal cancer cells. In addition, walnut green peel extract will also affect the expression levels of esophageal cancer cell-related genes.Keywords: Chinese medicine; walnut green peel; esophageal cancer; cell proliferation; molecular mechanism.

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中药核桃青皮提取物对食管癌细胞增殖的抑制作用及其分子机制的探讨的开题报告

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