精品文档---下载后可任意编辑靶向 hTERT 的肝特异性 RNAi 载体的构建与鉴定的开题报告摘要肝癌作为全球最常见的肿瘤之一，对人类健康造成了严重威胁。目前，治疗手段相对有限，因此探究新的治疗策略对于肝癌治疗至关重要。本讨论旨在构建一种能够靶向 hTERT 的肝特异性 RNAi 载体，以实现对肝癌的治疗。本讨论将通过基因克隆技术，构建 hTERT-siRNA 表达载体，并通过细胞实验和动物实验进行验证其特异性和有效性。估计本讨论将为探究肝癌治疗新策略提供有价值的参考。关键词：肝癌；hTERT；RNAi 载体；基因克隆技术；特异性AbstractAs one of the most common tumors in the world, liver cancer poses a serious threat to human health. Currently, the treatment options are relatively limited, so exploring new treatment strategies is crucial for liver cancer treatment. The aim of this study is to construct a liver-specific RNAi carrier that can target hTERT to achieve treatment for liver cancer. In this study, hTERT-siRNA expression vector will be constructed by gene cloning technology, and its specificity and effectiveness will be verified through cell experiments and animal experiments. It is expected that this study will provide valuable reference for exploring new strategies for liver cancer treatment.Keywords: liver cancer; hTERT; RNAi carrier; gene cloning technology; specificity
