病毒诱导的植物基因沉默详解

20安必奇生物科技有限公司90genesilencing,VIGSzvirus-inducedD1.DDDDDDDDDDDDVIGSVIGSRNARNAiRNAiVIGScDNADCLDCL4RNase-RNARIS2.double-strandedRNA,dsRNAdsRNADicerRNAsmallinteferingRNA,siRNARNA-inducedsilencingcomplexRISCRISCRNAmRNADNAmRNARNAsiRNAsArgonatuteAGORNARNase□IIHEHBrnmI=11mRNAoftargetgenesiRNA,21-22ntmRNAofla^etgeneTarget,gen*intheplantVKSSvectorRecpmlqinajitviralRIM 旷）-—OX>~日品尿㈠ReplicativeformAbberan«RNAVirslRNA 严）S9IAS9IAe乙S9IA0N 占•乙dq£乙dqoOG-OOC安必奇生物科技有限公司3.RNADNARNADNARNA4.5.VIGSRT-PCRqPCRVIGSVIGS1RNAVIGSRNATMVXPVXTRVBSMVPEBVCMVBMVTRVBMVVIGSCMV2.2DNADNACbLCV1134DNATGMVACMV2002STMVDNADNA1siRNAmRNAsiRNA-scan安必奇生物科技有限公司2.300-500bp1.5kb6[1].BSMVSBEIIaSSIIa[D].,2014.[2],,-[J]-(),2008,34(2):119-131.