摘 要本次实验目的是利用 MTT 法来检测滑菇培养基菌丝与琥珀酸脱氢酶（SDH）活性之间的关系，从而推出滑菇培养基在不同酸碱度时的菌丝生物量。实验以滑菇 5188 菌种为实验材料，首先通过筛选找到合适的培养基配方和菌丝培养 方 法 ， 其 次 将 液 体 培 养 好 的 滑 菇 菌 丝 球 分 别 称 取0.05g、0.1g、0.15g、0.2g、0.25g、0.3g、0.35g、0.4g 利用 MTT 法检测出琥珀酸脱氢酶（SDH）与滑菇菌丝之间的标准曲线，以石灰（0%、1%、2%、3%、4%、5%、6%）来调节固体培养基的酸碱度，然后以此曲线为标准得出滑菇固体培养基菌丝在不同酸碱条件下其菌丝生物量是否有变化。实验结果表明：石灰量为 1%-3%的区间，菌丝生物量总体呈上升趋势，在石灰量为 3%时达到最高。而当石灰量达到 4%及以上时，菌丝生物量有所下降。所以，培养基石灰含量 3%最适合菌丝生长。本次实验是为MTT 法测定食用菌菌丝生物量提供理论参考。关键词：滑菇；MTT；菌丝生物量；琥珀酸脱氢酶AbstractThe purpose of this experiment is to use the MTT method to detect the relationship between the mycelium of the mushroom medium and the activity of succinate dehydrogenase (SDH), so as to introduce the mycelial biomass of the mushroom medium at different pH levels. In the experiment, 5188 strains of sliding mushrooms were used as experimental materials. First, the suitable medium formulation and mycelium cultivation method were found through screening. Secondly, the liquid cultured sliding mushroom mycelium balls were weighed 0.05g, 0.1g, 0.15g, 0.2 respectively. g, 0.25g, 0.3g, 0.35g, 0.4g using the MTT method to detect the standard curve between succinate dehydrogenase (SDH) and the mushroom hyphae, with lime (0%, 1%, 2%, 3 %, 4%, 5%, 6%) to adjust the pH of the solid medium, and then use this curve as a standard to obtain whether the mycelial biomass of the mycelia of the solid medium of the pleurotus ostreatus has changed under different acid and alkali conditions. The experimental re...
