多酚的提取,纯化,定性及抗氧化性VIP免费

香蕉皮多酚的提取、纯化及抗氧化性实验摘要：香蕉为世界”四大水果”(荔枝、菠萝、椰子,香蕉）之一，富含糖类、果胶、K、Ca、Mg、维生素C、维生素E和β一胡萝卜素等营养成分以及黄酮类化合物、多酚氧化酶、挥发油等多种活性成分，具有较高的营养价值和保健作用。研究显示，香蕉皮中不仅含有多种营养成分，而且含有多种抗氧化活性成分，因而具有较大的开发和利用价值。目前，国内外对香蕉皮的利用形式主要为饲料或食用菌培养基，产品附加值不高，有关香蕉皮中多酚类物质的提取报道极少。因此，本文对香蕉皮中多酚的提取条件和纯化及其抗氧化性能进行了一定的研究。结果得出，香蕉皮中多酚的最佳提取组合为：乙醇浓度为80％，浸提次数为2次，温度为80℃，时间为3h。用乙酸乙酯纯化后，多酚含量达到了32．54％，纯化效果比较理想。通过对猪油抗氧化性的研究，得出了香蕉皮多酚具有很强的抗氧化能力的结论。关键词：香蕉皮；多酚；提取；定性；纯化；抗氧化I成都大学学士学位论文（设计）ExtractandPurifyPolyphenolfromBananaPeerandOxidationResistanceTestAbstract:Asoneofthe"fourkindsofrichestfruitintheworld"（litchi,pineapple,coconutBanana).Bananaisinrichofsugars,fruitGlue,K,Ca,Mg,vitaminC,vitaminEandp-carotene,etc.,andothernutrientsaswellasflavonoids,polyphenoloxidase,volatileoil,avarietyofactiveingredientswithhighnutritionalvalueandhealtheffects.Studiesshowsthat,bananapeernotonlycontainsavarietyofnutritions,butalsocontainsavarietyofantioxidantactivityingredients,whichhasgreatdevelopmentandusevalue.Currently,theuseofbananapeelathomeandabroadmainlyintheformoffeedandediblemedium,nothighvalue-addedproducts,meanwhile,thebananapeelintheextractionofpolyphenolsreportedrarely.Therefore,thispaperwilldevotetostudyingtheextractionconditionsandpurificationofpolyphenolinbananapeel.Theresultsshowthatthebestextractioncombinationofpolyphenolinbananapeelisthattheconcentrationofethanolis80%,timesofextractionis2,temperatureis80℃,timeis3h.Afterpurifyingbychloroformandethylacetate,thecontentofpolyphenolreaches32.54%.Theeffectofpurificationisideal.Byresearchingoxidationresistancecapabilityoflard,itisconcludedthatthebananapeerpolyphenolhavestrongantioxidantcapacity.Keywords:bananapeel;polyphenol;featuresidentification;extraction;purification;antioxidation.II成都大学学士学位论文（设计）目录绪论..............................................................................1一材料、试剂和仪器的介绍.......................................................31．1材料、试剂和仪器.............................................................31．1．1材料...................................................................31．1．2试剂和仪器.............................................................3二香蕉皮多酚的提取.............................................................42.1工艺流程.....................................................................42.2不同浓度乙醇对香蕉皮中多酚提取操作要点........................................42.2.1筛选.....................................................................42.2.2烫漂.....................................................................42.2.3切细.....................................................................42.2.4烘干.....................................................................42.2.5浸提.....................................................................42.2.6减压抽滤.................................................................42.2.7离心.....................................................................42.2.8...