中国组织工程研究与临床康复第15卷第50期2011–12–10出版JournalofClinicalRehabilitativeTissueEngineeringResearchDecember10,2011Vol.15,No.50ISSN1673-8225CN21-1539/RCODEN:ZLKHAH93851SouthernMedicalUniversity,Guangzhou510515,GuangdongProvince,China;2DepartmentofHumanAnatomy,SouthernMedicalUniversity,GuangdongProvincialKeyLaboratoryofTissueConstructionandInspection,Guangzhou510515,GuangdongProvince,ChinaZhouFang☆,Studyingfordoctorate,SouthernMedicalUniversity,Guangzhou510515,GuangdongProvince,ChinaLiJing,Studyingfordoctorate,SouthernMedicalUniversity,Guangzhou510515,GuangdongProvince,ChinaLiyu12399@126.comZhouFangandLiJingcontributedequallytothispaper.Correspondenceto:LiaoHua,Doctor,Professor,DepartmentofHumanAnatomy,SouthernMedicalUniversity,GuangdongProvincialKeyLaboratoryofTissueConstructionandInspection,Guangzhou510515,GuangdongProvince,ChinaCorrespondenceto:OuyangJun,Doctor,Professor,DepartmentofHumanAnatomy,SouthernMedicalUniversity,GuangdongProvincialKeyLaboratoryofTissueConstructionandInspection,Guangzhou510515,GuangdongProvince,Chinajouyang@126.com兔桡骨临界骨缺损模型的制备*☆◆周芳1，李静1，余磊2，廖华2，欧阳钧2EstablishmentofaradialbonecriticaldefectmodelinarabbitZhouFang1,LiJing1,YuLei2,LiaoHua2,OuyangJun2AbstractBACKGROUND:Nonunioncausedbyvariousreasonsoffracturesandbonedefectshasbeenamajorproblemofclinicalorthopedicrehabilitation.Withtherapiddevelopmentoftissueengineering,productqualitytestingneedsstandardbonedefectmodel.However,thelengthofbonecritical-sizedefectshasnotbeenascertained.OBJECTIVE:Toestablishrabbitradialbonedefectmodelsandtodeterminethelengthofrabbitradialbonecritical-sizedefects.METHODS:EighteenmaleNewZealandwhiterabbits(2.0-2.5kg)wererandomlyassignedinto6groupsandthen12,13,14,15,16and17mmsegmentaldefectswerecreatedinthemiddlepartofradiusonbothsides,respectively.Theincisionsweresuturedandbandaged,butnotfixed.RESULTSANDCONCLUSION:AccordingtoexaminationwithnakedeyesandX-ray,someofthesegmentaldefectswiththelengthof12,13,14,15and16mmwerecompletelyrepaired.Butnoneofthedefectswiththelengthof17mmwasrepaired.Histologicalexaminationshowedthattherewastrabecularboneformation,bonematrix,medullarycavityrecanalization,revascularizationandosteoblastsinthesegmentaldefectswiththelengthof12,13,14,15and16mm.However,therewereosteoblasts,osteoclastsinthe17mmdefects,butnomedullarycavityrecanalizationorrevascularization.Therefore,thelengthofbonecritical-sizedefectinrabbitradiuswas17mm.ZhouF,LiJ,YuL,LiaoH,OuyangJ.Establishmentofaradialbonecriticaldefectmodelinarabbit.ZhongguoZuzhiGongchengYanjiuyuLinchuangKangfu.2011;15(50):9385-9388.[http://www.crter.cnhttp://en.zglckf.com]摘要背景：各种原因造成的骨折和骨缺损导致的骨不连一直是临床骨科修复的一大难题，相关产品质量的检验需要标准的骨缺损模型，然而骨缺损的临界长度至今仍无定论。目的：建立兔桡骨骨缺损模型，以确定兔桡骨临界骨缺损长度。方法：将18只雄性新西兰大白兔，随机分为6组，在双侧桡骨中段分别做12，13，14，15，16，17mm的缺损，伤口缝合包扎但不固定。结果与结论：大体标本和放射学观察显示，3个月内12，13，14，15，16mm组的缺损均有修复完整的情况，但17mm组无一例修复。组织学结果显示12，13，14，15，16mm组缺损修复区有骨小梁与骨基质的形成、骨再生和再血管化、髓腔不同程度再通以及成骨细胞，17mm组可见成骨细胞，破骨细胞，但未见骨再血管化和髓腔再通。故兔桡骨临界骨缺损长度为17mm。关键词：桡骨；骨缺损；临界骨缺损；长度；兔doi:10.3969/j.issn.1673...
