电针对偏头痛大鼠的皮层扩展性抑制以及对血浆降钙素基因相关肽_P物质含量的影响VIP免费

项目来源:国家自然科学基金资助(30901932)通讯作者:景向红,女,研究员,主要从事针灸作用机制研究工作。Tel:(010)64014411转2750电针对偏头痛大鼠的皮层扩展性抑制以及对血浆降钙素基因相关肽、P物质含量的影响石宏李江慧吉长福尚红艳邱恩超1王京京景向红(中国中医科学院针灸研究所,北京100700;1解放军总医院神经内科,北京100853)【摘要】目的:观察针刺对偏头痛大鼠皮层扩展性抑制(CSD)电位以及体内降钙素基因相关肽(CGRP)及P物质(SP)含量的影响,探讨针刺治疗偏头痛的机制。方法:将SD大鼠随机分为正常组、模型组和电针组,每组10只。模型组在皮层顶叶局部给予3mol/LKCl诱导CSD,在额叶放置记录电极;电针组在皮层施予3mol/LKCl的同时电针双侧“阳陵泉”“太冲”穴30min。观察偏头痛大鼠在电针前后CSD的波幅变化,放射免疫法测定大鼠血浆中CGRP及SP含量的变化。结果:模型组给予KCl后,经过3min左右诱导出CSD,表现为负向电位,幅度为(-25113±1123)mV,电针组经电针治疗可明显抑制CSD的波幅,平均幅度为(-19119±1153)mV,二者相比差异有统计学意义(P<0101);电针抑制CSD波幅的作用可持续50min;模型组大鼠的血浆中CGRP含量明显增高,与正常组相比差异有统计学意义(P<01001),而经过电针治疗的偏头痛大鼠血浆中CGRP含量较模型组明显降低(P<0105)。模型组血浆中的SP含量与正常组相比显著升高(P<0101),经电针治疗后大鼠血浆中SP含量较模型组显著下降(P<01001)。结论:电针“阳陵泉”和“太冲”可以显著抑制偏头痛大鼠CSD的波幅,降低大鼠血浆中的CGRP和SP含量,提示电针对偏头痛的治疗作用可能是通过调节血浆中的CGRP及SP含量实现的。【关键词】偏头痛皮层扩展性抑制电针降钙素基因相关肽P物质【中图分类号】R24519+7【文献标志码】A文章编号:100020607(2010)0120017205EffectofElectroacupunctureonCorticalSpreadingDepressionandPlasmaCGRPandSubstancePContentsinMigraineRatsSHIHong,LIJiang2hui,JIChang2fu,SHANGHong2yan,QIUEn2chao1,WANGJing2jing,JINGXiang2hong(InstituteofAcu2moxibustion,ChinaAcademyofChineseMedicalSciences,Beijing100700,China;1DepartmentofNeu2rology,GeneralHospitalofthePeople’sLiberationArmy,Beijing100853)【ABSTRACT】ObjectiveToobservetheeffectofelectroacupuncture(EA)oncorticalspreadingdepression(CSD)andcontentsofplasmacalcitoningene2relatedpeptide(CGRP)andsubstanceP(SP)inmigrainerats.MethodsThirtymaleSDratswereequallyrandomizedintocontrol,modelandEAgroups.MigrainemodelwasestablishedbytopicalapplicationofKCl(3mol/L)immersedinapieceoffilterpapertothecerebralcortex(parietallobe,6mmposteriortotheBregmaand5mmtothesagitalfissure)afterexposureoftheskull(inreferencetoMichael’meth2od).KClstimulationevokedCSDpotentials(3mmrostraltotheBregma,and2mmtothesagitalfissure)werere2cordedbyusingaglassmicroelectrode.Forratsofcontrolgroup,filterpapercontaining019%NaClwasappliedtothesameparietalcortexarea.EA(1mA,2Hz/100Hz)wasappliedtobilateral“Yanglingquan”(GB34)and“Tai2chong”(LR3)for30min.ThecontentsofplasmaCGRPandSPwereassayedbyradioimmunoassay.ResultsCSDwasinduced3-5minafterapplicationofKCltotheparietallobe.Theaverageamplitudeofmodelgroupwas(-25113±1123)mV,andthatofEAgroupwas(-19119±1153)mV,displayingasignificantre2ductionofCSDamplitudeafterEA(P<0101).Comparisonamong3groupsshowedthatbothplasmaCGRPandSPcontentsinmodelgroupweresignificantlyhigherthanthoseofcontrolgroup(P<01001,P<0101),whilecomparedwithmodelgroup,plasmaCGRPandSPlevelsinEAgroupdecreasedconsiderably(P<0105,P<01001),suggestinganinhibitoryeffectofEAonpain2producingsubstance.ConclusionEAof·71·针刺研究2010年2月第35卷第1期GB34andLR3caneffectivelysuppressKClprovokedcorticalspreadingdepressionandplasmaCGRPandSPlevelsintherat,whichmaycontributetoitseffectinrelievingmig...