垦!!!!!!!!!翌!!堕堕!!旦!!g!!!!!：!!!：!!堕!：i一’’’’’’’’’’’、：实验研究：、Il‘III‘tII，主国堑垫苤查!!!!生筮!!鲞箜!塑软脉灵对动脉粥样硬化鹌鹑血浆内皮素和降钙素基因相关肽的影响丁选胜1，叶明2，胡哲一1，安娜芹1(1中国药科大学药理研究室，南京210009；2福建新大陆药业有限公司，福州350003)[摘要]目的：观察软脉灵对动脉粥样硬化鹌鹑血浆内皮素和降钙素基因相关肽的影响。方法：雄性日本鹌鹑60只，随机均分成6组，即正常组、动脉粥样硬化模型组、软脉灵低、中、高剂量(折合成生药7．5，15和30g·kg“)组和阳性对照普伐他汀钠8mg·kg。1组，每组i0只。除正常组外其余5组鹌鹑给予高脂饲料16周制备动脉粥样硬化模型，给药组在造模的同时，灌胃给药，qd，持续16周。16周后采血，测血浆内皮素和降钙素基因相关肽以及血清胆固醇和三酰甘油的含量；并取主动脉，观察斑块分级及主动脉内膜厚度，制作病理切片。结果：与正常对照组比较，模型组动物在喂饲高脂饲料16周后主动脉内膜厚度显著增加，动脉粥样硬化斑块形成；其血浆内皮素升高，降钙素基因相关肽降低，血清总胆固醇和三酰甘油明显升高。与模型组比较，软脉灵组剂量依赖性的减轻动脉粥样硬化斑块的形成和主动脉内膜的厚度，降低血浆内皮素及血清总胆固醇和三酰甘油，升高降钙素基因相关肽。结论：软脉灵抗动脉粥样硬化的机制可能与其降低血浆内皮素和升高降钙素基因相关肽有关。[关键词]软脉灵；动脉粥样硬化；肉皮素；降钙素基因相关肽[中图分类号]R285．5；R286．2[文献标识码]A[文章编号]1003—3734(2007)03—0216—03EffectsofRuanmailingOilplasmaendothelinandcalcitoningene-relatedpeptidesinquailatherosclerosismodelDINGXuan—shen91，YEMin92，HUZhe—yil，ANNa—qinl(1ResearchSectionofPharmacology，ChinaPharmaceuticalUniversity，Na，讲ng210009，China；2FufianNewlandPharmaceuticalCo．，Ltd．，Fuzhou350003，China)[Abstract]Objective：ToevaluatetheeffectsofRuanmailingintheimprovementsofplasmaen—dothelinandealcitoningene—relatedpeptides(CGRP)inquailatherosclerosismodel．Methods：60Jap·anesemalequailswererandomlyassignedintosixgroups(n=10each)：normalhealthyquails，athero—sclerosisquails，atherosclerosisquailssupplementedwithRuanmailing(7．5，15or30g·kg“)orpo—ravastatin8mg·kg～．Theatherosclerosismodelofquailswasestablishedbyfeedinghighfatdietsfor16weeks．Thequailsthatwerebeinginducedtheatherosclerosisweresimuhaneouslyadministeredwiththedrugsdailyfor16weeks。Attheendoftheadministration。thebloodsampleswerecollectedtomeasuretheplasmaconcentrationsofendothelinandCGRP，andtheserumconcentrationsoftotalcholesterol(TC)andtriglyceride(TG)．Thequailswerethensacrificedtocollecttheaortaeforthedeterminationofthethicknessofendomembraneandatherosclerosticplaque。Results：Comparedwiththenormalhealthyquails，thequailswithatherosclerosishadthickeraorticendomembraneandmoremorbidatherosclerosticplaque．Theatherosclerosticquailsshowedincreasesofendothelin，TCandTGlevels，andadecreaseofCGRPlevel．Comparedwiththeatherosclerosticquails，theRuanmailing—treatedquailsexperiencedthereducedatheroselerostic"plaqueandaorticendomembranethickness，thedecreasesofendothelin，TCandTGlevels，andanescalationofCGRP1evelsinadose—dependentmanner．Conclusion：Ruanmailingal—leviatedtheatherosclerosisbydecreasingtheendothelinandincreasingtheCGRP．[Keywords]Ruanmailing；atherosclerosis；endothelin；ealeitoningene—relatedpeptide(CGRP)软脉灵口服液是由熟地黄、五味子、枸杞子、制何首乌、白芍、怀牛膝、炙黄芪、人参、淫羊藿、当归、川芎、丹参等药物组成的纯中药复方制剂，具有滋补肝一216一肾、益气活血之功。该制剂对防治动脉粥样硬化及其相关疾病有一定疗效。本研究观...
