红色诺卡氏菌的生物活性张祝兰* 唐文力 黄颖桢 洪金基(福建省微生物研究所 福建 福州 350007)摘 要: 为了考察红色诺卡氏菌菌体(NC)的生物活性, 通过一定浓度的 NC 对小鼠灌胃给药, 检测其毒性及对免疫器官、巨噬细胞(MΦ)吞噬功能的影响和对肉瘤 S180 抑制作用。结果表明小鼠口服NC, LD50>10 g/kg; NC 明显增加小鼠胸腺脾脏重量、提升白细胞数量和提高小鼠 MΦ 的吞噬活性; 对小鼠腹腔 MΦ 具有明显的激活作用, 激活了的 MΦ 能增强抑杀白色念珠菌作用, 正常的小鼠 MΦ也有一定的杀菌作用, 两者差异显著; 对小鼠 S180腹水型转实体瘤具有明显的抑制作用。由此得出的结论是, NC 毒性低, 能显著增强机体免疫功能, 具有抗病原微生物感染及明显的抑瘤作用。关键词: 红色诺卡氏菌, 免疫, 抑瘤, 生物活性Bioactivity of Nocardia rubra CellZHANG Zhu-Lan* TANG Wen-Li HUANG Ying-Zhen HONG Jin-Ji(Fujian Provinical Institute of Microbiology, Fuzhou, Fujian 350007, China)Abstract: To investigate the bioactivity of Nocardia rubra Cell (NC), the mice were used to assay the toxicity, the effects on immune organs, phagocytes of peritoneal macrophage and the antitumor activity by perfusion of NC to the stomach of mice. Results indicated that NC could obviously stimulate in vitro the phagocytosis of peritoneal macrophage from mice, and remarkably inhibit the growth of S 180 in mice, and its LD50 was more than 10 g/kg. In conclusion, NC had low toxicity, it could significantly enhance the organism immunologic function and had obvious antitumor effect and the anti-infection effect against a pathogenic microorganism.Keywords: Nocardia rubra Cell, Immunity, Antitumor, Bioactivity在从微生物筛选生物活性物质过程中, 筛选到一株红色诺卡氏菌 Nocardia rubra 03-PO-8[1], 其细胞壁骨 架 (Nocardia rubra cell wall skeleton, N-CWS) 与BCG、CP、干扰素、胸腺因子 D 等同属生物反应调节剂, 已证实 N-CWS 能激活机体免疫功能, 增强免疫细胞的活性, 诱生干扰素等细胞因子[2,3], 适用于各种肿瘤引...
