下载后可任意编辑响应面优化乳杆菌 PC-3 菌株产共轭亚油酸的工艺讨论摘 要: 以共轭亚油酸(CLA)转化率为考察指标, 讨论乳杆菌 PC-3 菌株转化生产CLA 的发酵工艺。在单因素试验中考察培育基种类、 乳糖与硫酸铵添加量比、 菌液接种量、 培育时间、 亚油酸添加量、 吐温-80 添加量对 CLA转化率影响的基础上, 采纳三因素三水平响应面试验设计优化乳杆菌 PC-3 菌株产共轭亚油酸的工艺条件。结果表明: 乳杆菌 PC-3 菌株产共轭亚油酸的较佳条件为: 诱导培育基为 MRS 培育基、 乳糖与硫酸铵添加量比 3:2、 接种量 3.0%、 培育时间 43.0h、 亚油酸(LA)添加量(体积分数)1.30‰、 乳化剂吐温-80 添加量 2.00g/L, 此条件下 CLA 转化率达到 6.87%, 理论值可达6.96%, 实验值与理论预测值的相对误差为 1.29%。关键词: 共轭亚油酸; 乳杆菌 PC-3; 响应面分析法; 优化Optimization of Medium Composition and Culture Conditions for Conversion of Linoleic Acid toConjugated Linoleic Acid by Lactobacillus PC-3Abstract: Themediumcompositionandcultureconditionsforconversionoflinoleicacid(LA)toconjugatedlinoleicacid(CLA)byLactobacillusPC-3wereoptimizedbyresponsesurfacemethodologytoobtainmaximumconversionefficiencyofCLA.Culturemediumtype,lactose/ammoniumsulfateconcentrationratio,inoculumsamount,culturetime,LAconcentrationandTween-80concentrationwereinvestigatedbyone-factor-at-a-timeexperiments.Athree-variable,three-levelresponse下载后可任意编辑surfacedesignwasusedtooptimizeinoculumamount,culturetimeandLAconcentration.TheresultsshowedthattheoptimalconversionconditionsofLAwasMRSmedium,inoculumsamountof3.0%,culturetimeof43.0h,LAconcentrationof1.30‰andTween-80concentrationof2.00g/L.Undertheoptimizedconditions,theexperimentalconversionefficiencyofCLAwas6.87% compared to 6.96% from theoretical predictions, showing a relative error of 1.29% between both.Key words: conjugated linoleic acid; LactobacillusPC-3; response surface methodology; optimization中图分类号: Q815文献标志码: A 文章编号: 1002-6630( )19-0213-06doi:10.7506/spkx1002-6630- 19044收稿日期: -07-30基金项目: ...
