精品文档---下载后可任意编辑CTLA4Ig 及整合素 α4β7 基因重组腺病毒修饰树突状细胞的开题报告摘要：细胞免疫治疗近年来受到广泛关注，而树突状细胞（DCs）是一类十分重要的抗原呈递细胞。为了提高树突状细胞的治疗效果，本讨论计划利用重组腺病毒载体对CTLA4Ig 及整合素 α4β7 基因进行修饰，并将其转染至树突状细胞中。通过流式细胞术及实时荧光定量 PCR 对转染后的细胞进行检测，以确定修饰的效果。此讨论的开展将为进一步探究树突状细胞的治疗效果及机制奠定基础。关键词：树突状细胞；CTLA4Ig；整合素 α4β7；重组腺病毒；转染Abstract:Cellular immunotherapy has received extensive attention in recent years, and dendritic cells (DCs) are an important group of antigen-presenting cells. In order to improve the therapeutic effect of dendritic cells, this study plans to use recombinant adenovirus vectors to modify the CTLA4Ig and integrin α4β7 genes and transfect them into dendritic cells. The modification effect will be determined by flow cytometry and real-time fluorescent quantitative PCR analysis of the transfected cells. The development of this study will lay a foundation for further exploring the therapeutic effect and mechanism of dendritic cells.Keywords: dendritic cells; CTLA4Ig; integrin α4β7; recombinant adenovirus; transfection
