精品文档---下载后可任意编辑HIBD 新生大鼠脑皮质及海马 OPC 死亡通路的讨论的开题报告摘要：本讨论旨在探究 HIBD 新生大鼠脑皮质及海马 OPC 死亡通路的机制。采纳分子生物学、免疫组化等方法，通过观察 HIBD 新生大鼠的脑组织中OPC 的变化，分析发现 HIBD 能够导致脑组织中 OPC 数量的减少，而导致 OPC 减少的原因是由于 HIBD 的作用导致 OPC 凋亡。因此，本讨论将主要讨论在 HIBD 新生大鼠脑皮质及海马中 OPC 死亡通路的机制。首先，将采纳 TUNEL 检测法、实时荧光定量PCR（qRT-PCR）和 Western blot 技术来检测 OPC 凋亡相关基因和蛋白的表达变化；其次，利用药理学干预讨论 U0126 和 PD98059 对HIBD 新生大鼠脑组织中 OPC 凋亡的影响；最后，采纳细胞培育、RNA干扰等技术探究 HIBD 对 OPC 的影响及潜在机制。讨论结果将有助于深化了解 HIBD 对 OPC 凋亡的影响及机制，并为其临床治疗提供新的思路和方法。关键词：脑缺氧缺血性损伤（HIBD）；脑皮质；海马；Oligodendrocyte precursor cells（OPC）；凋亡通路Abstract:The aim of this study is to investigate the mechanism of oligodendrocyte precursor cells (OPC) death pathway in the cortex and hippocampus of HIBD neonatal rats. Molecular biology, immunohistochemistry and other methods were used to observe the changes of OPC in the brain tissue of HIBD neonatal rats, and it was found that HIBD can lead to a decrease in the number of OPC in brain tissue, which is caused by the apoptosis of OPC induced by HIBD.Therefore, this study will mainly investigate the mechanism of OPC death pathway in the cortex and hippocampus of HIBD neonatal rats. First, TUNEL detection, real-time fluorescent quantitative PCR (qRT-PCR) and Western blot technology will be used to detect the expression changes of apoptosis-related genes and proteins in OPC; Secondly, pharmacological intervention studies U0126 and PD98059 will be used to study the effect of OPC apoptosis in brain tissue of HIBD neonatal rats; Finally, cell culture, RNA interference and 精品文档---下载后可任意编辑other technologies will be used to investigate the effect of HIBD on OPC and its potential mechanism.The results of this study will help to understand the effect and mechanism of HIBD on OPC apoptosis, and provide new ideas and methods for its clinical treatment.Keywords: hypoxic-ischemic brain damage (HIBD); cortex; hippocampus; oligodendrocyte precursor cells (OPC); apoptosis pathway
